Papasian C J, Hertlein G
Laboratory Service, Veterans Administration Medical Center, Kansas City, MO 64128.
Diagn Microbiol Infect Dis. 1987 Dec;8(4):255-8. doi: 10.1016/0732-8893(87)90058-7.
We evaluated three fluorogenic methods (MUG; Remel, Lenexa, KS) for the rapid (less than 1 hr) identification of E. coli by detecting beta-glucuronidase activity. The methods included: direct disk inoculation test, tube test, and liquid spot reagent test. Fluorogenic tests were performed on pure cultures of lactose fermenters and compared with identification by Enterotube II (Roche Diagnostics; Nutley, NJ). Organisms yielding disparate results were further analyzed by API 20E (Analytab Products Inc.; Plainview, NY). The tube test was evaluated with isolates subcultured on both MacConkey and blood agars; the direct disk and liquid spot reagent methods were tested with isolates subcultured on blood agar only. All methods were highly specific (greater than 97%). Sensitivity of the beta-glucuronidase tests were method and media dependent, but exceeded 85% in all cases. The direct disk and tube tests also permitted detection of indole formation; results of indole testing, however, contributed little to accurately identifying E. coli.
我们评估了三种荧光法（MUG；Remel公司，莱尼克斯，堪萨斯州），通过检测β-葡萄糖醛酸酶活性来快速（少于1小时）鉴定大肠杆菌。这些方法包括：直接平板接种试验、试管试验和液体斑点试剂试验。对乳糖发酵菌的纯培养物进行荧光试验，并与使用肠杆菌鉴定管II（罗氏诊断公司；新泽西州纳特利）进行的鉴定结果相比较。对产生不同结果的菌株进一步使用API 20E（Analytab Products公司；纽约州普莱恩维尤）进行分析。试管试验用接种在麦康凯琼脂和血琼脂上的分离株进行评估；直接平板法和液体斑点试剂法仅用接种在血琼脂上的分离株进行测试。所有方法的特异性都很高（大于97%）。β-葡萄糖醛酸酶试验的灵敏度取决于方法和培养基，但在所有情况下均超过85%。直接平板法和试管试验还可以检测吲哚的形成；然而，吲哚试验结果对准确鉴定大肠杆菌的作用不大。
