使用荧光培养基从尿液中快速鉴定大肠杆菌。

Rapid identification of Escherichia coli from urine by using Fluorocult media.

作者信息

Mori T, Takahashi H, Maehata E, Naka H

机构信息

Central Clinical Laboratories, Mitsui Memorial Hospital, Tokyo, Japan.

出版信息

Acta Microbiol Hung. 1991;38(3-4):273-81.

PMID:1817424

Abstract

For rapid identification of Escherichia coli, we evaluated Fluorocult MacConkey Agar, Fluorocult Laurylsulfate Broth and Bactident E. coli, which are incorporating fluorogenic substrate, MUG (4-methylumbeliferyl-beta-D-Glucuronide) that specifically reacts with E. coli. To assess the specificity and sensitivity of Fluorocult MacConkey Agar and Laurylsulfate Broth, beta-D-glucuronidase; beta-GUR activities of 264 strains from urine including 72 of E. coli were investigated. For both media, sensitivity was 92% and specificity was 100%. When there was 10(8) c.f.u./ml of E. coli in urine specimen, incubation times required for positive fluorescence by Fluorocult MacConkey Agar, Laurylsulfate Broth, and Bactident E. coli were 8 h, 4 h and 15 min, respectively. Influence of drugs in urine to fluorescence reaction was not observed.

摘要

为了快速鉴定大肠杆菌，我们评估了含荧光底物MUG（4-甲基伞形酮-β-D-葡萄糖醛酸苷）的Fluorocult麦康凯琼脂、Fluorocult月桂基硫酸盐肉汤和Bactident大肠杆菌检测试剂，该荧光底物能与大肠杆菌发生特异性反应。为评估Fluorocult麦康凯琼脂和月桂基硫酸盐肉汤的特异性和敏感性，我们研究了来自尿液的264株菌株（包括72株大肠杆菌）的β-D-葡萄糖醛酸酶；β-GUR活性。两种培养基的敏感性均为92%，特异性均为100%。当尿液标本中大肠杆菌浓度为10⁸ c.f.u./ml时，Fluorocult麦康凯琼脂、月桂基硫酸盐肉汤和Bactident大肠杆菌检测试剂产生阳性荧光所需的孵育时间分别为8小时、4小时和15分钟。未观察到尿液中的药物对荧光反应的影响。