Institute of Animal Husbandry and Veterinary Science, Henan Academy of Agricultural Sciences, Zhengzhou, 450002, China.
Key Laboratory of Animal Immunology of the Ministry of Agriculture, Henan Academy of Agricultural Sciences, Zhengzhou, 450002, China.
Proteomics. 2021 Jan;21(2):e2000019. doi: 10.1002/pmic.202000019. Epub 2020 Dec 21.
Porcine reproductive and respiratory syndrome (PRRS), caused by PRRS virus (PRRSV), is a serious viral disease affecting global swine industry. Due to the lack of effective vaccines, new antiviral strategies to compensate for the inefficacy of available vaccines are urgently required. Lysine acetylation, as an important post-translational modification during infection, plays a key regulatory role in host antiviral responses. In this study, the global acetylome is profiled using acetylation specific antibody-based enrichment and tandem mass tag label high-affinity purification liquid chromatography-mass spectrometry in PRRSV-infected pulmonary alveolar macrophages (PAMs). As a result, 3731 lysine acetylation sites on 1421 cellular proteins are identified. Bioinformatics analysis of the different acetylated proteins revealed their involvement in various biological processes, including the host immune response and energy metabolism. These findings will contribute to the understanding of PRRSV pathogenesis and identify new cellular targets for anti-PPRSV therapeutics.
猪繁殖与呼吸综合征（PRRS）由猪繁殖与呼吸综合征病毒（PRRSV）引起，是一种严重影响全球养猪业的病毒性疾病。由于缺乏有效的疫苗，急需寻找新的抗病毒策略来弥补现有疫苗的无效性。赖氨酸乙酰化作为感染过程中一种重要的翻译后修饰，在宿主抗病毒反应中发挥着关键的调控作用。在这项研究中，我们采用基于乙酰化特异性抗体的富集和串联质量标签标记高亲和力纯化液相色谱-质谱联用技术，对 PRRSV 感染的肺泡巨噬细胞（PAMs）进行了全乙酰组学分析。结果鉴定到 1421 种细胞蛋白上的 3731 个赖氨酸乙酰化位点。对不同乙酰化蛋白的生物信息学分析表明，它们参与了多种生物学过程，包括宿主免疫反应和能量代谢。这些发现将有助于理解 PRRSV 的发病机制，并为抗 PRRSV 治疗药物的研发提供新的细胞靶点。
