Department of Plant Breeding, Faculty of Crop Sciences, Sari Agricultural Sciences and Natural Resources University (SANRU), Sari 4818166996, Iran.
Department of Crop and Animal Production, Cilimli Vocational School, Duzce University, Duzce 81750, Turkey.
Genes (Basel). 2020 Dec 7;11(12):1464. doi: 10.3390/genes11121464.
Members of the / transcription factor family play critical roles in plant development, biosynthesis of key metabolites, and stress response. A detailed study was performed to identify ss in the durum wheat ( ssp. ) genome, which resulted in the identification of 271 genes distributed on chromosomes 1A-7B. By carrying 27 genes, chromosome 6A had the highest number of ss. Furthermore, a duplication assay of ss demonstrated that 70 duplicated gene pairs had undergone purifying selection. According to RNA-seq analysis, the highest expression levels in all tissues and in response to stimuli were associated with and subfamily genes. In addition, the results revealed that genes have tissue-specific expression patterns, and most genes were significantly induced in the root tissue. Additionally, 13 genes (six s, three s, two s, one , and one ) were selected for further analysis via qRT-PCR of their potential in coping with drought and salinity stresses. The genes belonging to the DREB subfamily were markedly induced under both drought-stress and salinity-stress conditions. Furthermore, docking simulations revealed several residues in the pocket sites of the proteins associated with the stress response, which may be useful in future site-directed mutagenesis studies to increase the stress tolerance of durum wheat. This study could provide valuable insights for further evolutionary and functional assays of this important gene family in durum wheat.
转录因子家族成员在植物发育、关键代谢物的生物合成和应激反应中发挥着关键作用。本研究旨在鉴定硬粒小麦（ ssp. ）基因组中的 ss，共鉴定到 271 个基因，分布在 1A-7B 染色体上。6A 染色体携带的 ss 数量最多，有 27 个基因。此外，对 ss 的复制分析表明，70 对复制基因经历了纯化选择。根据 RNA-seq 分析，所有组织和响应刺激的最高表达水平与 和 亚家族基因相关。此外，结果表明， 基因具有组织特异性表达模式，大多数 基因在根组织中显著诱导表达。此外，通过对 13 个 基因（6 个 s、3 个 s、2 个 s、1 个 、1 个 ）进行 qRT-PCR 分析，研究其在应对干旱和盐胁迫方面的潜在功能。属于 DREB 亚家族的 基因在干旱胁迫和盐胁迫条件下均明显诱导表达。此外，对接模拟揭示了与应激反应相关的蛋白口袋部位的几个残基，这可能有助于未来的定点突变研究，以提高硬粒小麦的抗胁迫能力。本研究可为硬粒小麦中这一重要基因家族的进一步进化和功能研究提供有价值的见解。
