Huang Youmei, Liu Yanhui, Zhang Man, Chai Mengnan, He Qing, Jakada Bello Hassan, Chen Fangqian, Chen Huihuang, Jin Xingyue, Cai Hanyang, Qin Yuan
State Key Lab of Ecological Pest Control for Fujian and Taiwan Crops; Key Lab of Genetics, Breeding and Multiple Utilization of Crops, Ministry of Education; Fujian Provincial Key Lab of Haixia Applied Plant Systems Biology, College of Life Sciences, Fujian Agriculture and Forestry University, Fuzhou, Fujian Province, China.
State Key Laboratory for Conservation and Utilization of Subtropical Agro-Bioresources, Guangxi Key Lab of Sugarcane Biology, College of Agriculture, Guangxi University, Nanning, Guangxi Province, China.
PeerJ. 2020 Sep 22;8:e10014. doi: 10.7717/peerj.10014. eCollection 2020.
Pineapple ( (L.) Merr.) is an important tropical fruit with high economic value. The quality and yield of pineapple will be affected by various environmental conditions. Under adverse conditions, plants can produce a complex reaction mechanism to enhance their resistance. It has been reported that the member of ethylene responsive transcription factors (ERFs) plays a crucial role in plant developmental process and stress response. However, the function of these proteins in pineapple remains limited. In this study, a total of 74 genes ) were identified in pineapple genome, named from to , and divided into 13 groups based on phylogenetic analysis. We also analyzed gene structure, conserved motif and chromosomal location of , and the within the same group possess similar gene structures and motif compositions. Three genes (, and ) were present on unanchored scaffolds, so they could not be conclusively mapped on chromosome. Synteny and -elements analysis of genes provided deep insight into the evolution and function of pineapple genes. Furthermore, we analyzed the expression profiling of in different tissues and developmental stages, and 22 genes were expressed in all examined tissues, in which five genes (, , , , and ) had high expression levels. Additionally, nine genes were selected for functional verification by qRT-PCR. These results provide useful information for further investigating the evolution and functions of ERF family in pineapple.
菠萝（(L.) Merr.）是一种具有高经济价值的重要热带水果。菠萝的品质和产量会受到各种环境条件的影响。在不利条件下，植物会产生复杂的反应机制以增强自身抗性。据报道，乙烯响应转录因子（ERFs）成员在植物发育过程和胁迫响应中起关键作用。然而，这些蛋白质在菠萝中的功能仍较为有限。在本研究中，在菠萝基因组中共鉴定出74个基因，命名为从 到 ，并基于系统发育分析将其分为13组。我们还分析了 的基因结构、保守基序和染色体定位，同一组内的 具有相似的基因结构和基序组成。三个基因（ 、 和 ）位于未锚定的支架上，因此无法最终定位到染色体上。 基因的共线性和 -元件分析为深入了解菠萝 基因的进化和功能提供了线索。此外，我们分析了 在不同组织和发育阶段的表达谱，22个 基因在所有检测组织中均有表达，其中五个基因（ 、 、 、 和 ）表达水平较高。此外，选择了九个 基因通过qRT-PCR进行功能验证。这些结果为进一步研究菠萝中ERF家族的进化和功能提供了有用信息。
