De Toni Luca, Muscianisi Francesco, Corsini Christian, Ghezzi Marco, Di Nisio Andrea, Foresta Carlo, Garolla Andrea
Unit of Andrology and Reproductive Medicine, Section of Endocrinology, Department of Medicine, Centre for Male Gamete Cryopreservation, University of Padova, 35122 Padova, Italy.
Vaccines (Basel). 2020 Dec 7;8(4):743. doi: 10.3390/vaccines8040743.
Persistent human papillomavirus (HPV) semen infection is increasingly associated with male infertility. Adjuvant HPV vaccination is suggested to reduce the time to clearance and the disease relapse in males with persistent HPV semen infection. However, only a sub-population of patients show a clinical benefit from adjuvant vaccination. Here, we aimed to address the effectiveness rate of HPV adjuvant vaccination in males with genital tract infection and the possible prognostic markers of healing. Clinical records from 379 patients with persistent seminal HPV detection, all receiving HPV adjuvant vaccination, were considered. Clinical data, including genital HPV-DNA assessment by INNO-LiPA genotyping, semen HPV-DNA analysis by FISH analysis and serum antibody titer, were collected at basal (T0) and after 6 months (T1) since the vaccination cycle ended. Clearance of genital HPV-DNA was recorded in 326 (86%) patients. Serum HPV-antibody titer at T1 was the most important prognostic factor associated with HPV-DNA clearance. A serum antibody titer equal to or greater than the threshold value 1:125, obtained by ROC curve analysis, was prognostic of healing. Anti-HPV antibody represents a suitable marker of adequate immune response to HPV vaccination in patients with genital infection.
持续性人乳头瘤病毒（HPV）精液感染与男性不育的关联日益密切。建议进行HPV辅助疫苗接种，以缩短持续HPV精液感染男性的病毒清除时间并降低疾病复发率。然而，只有部分患者能从辅助疫苗接种中获得临床益处。在此，我们旨在探讨HPV辅助疫苗接种对生殖道感染男性的有效性以及可能的愈合预后标志物。我们研究了379例持续检测到精液HPV且均接受HPV辅助疫苗接种患者的临床记录。收集了包括采用INNO-LiPA基因分型法评估生殖道HPV-DNA、采用荧光原位杂交（FISH）分析法分析精液HPV-DNA以及血清抗体滴度等临床数据，这些数据在疫苗接种周期结束后的基线期（T0）和6个月后（T1）收集。326例（86%）患者的生殖道HPV-DNA实现了清除。T1期的血清HPV抗体滴度是与HPV-DNA清除相关的最重要预后因素。通过ROC曲线分析得出的血清抗体滴度等于或高于阈值1:125可作为愈合的预后指标。抗HPV抗体是生殖器感染患者对HPV疫苗接种产生充分免疫反应的合适标志物。
