Reibnegger G, Fuchs D, Hausen A, Schmutzhard E, Werner E R, Wachter H
Institute of Medical Chemistry and Biochemistry, University of Innsbruck, Austria.
Trans R Soc Trop Med Hyg. 1987;81(5):729-33. doi: 10.1016/0035-9203(87)90009-5.
In vitro, neopterin, a pyrazino-[2, 3-d]-pyrimidine compound, is produced by human monocytes-macrophages following induction by either supernatants from activated T lymphocytes or by recombinant gamma interferon. In vivo, its determination in urine or serum provides a sensitive and specific test for the activation grade of cell-mediated immune reactions. Urinary neopterin levels were measured in 128 Tanzanian individuals (age 6 months to 54 years) with parasitologically proven malaria. Levels in a subgroup of 117 previously untreated patients were compared with those previously reported from 19 untreated malarial patients from Bangkok, Thailand (age 7 to 62 years). The influence of concomitant variables such as age, fever, parasitaemia, duration of symptoms and local endemicity of malaria upon neopterin excretion levels was analysed. In the Thai patients, levels were considerably higher than in Tanzanian subjects of similar age. Among the Tanzanian patients, an overwhelming influence of age was detected, children showing extremely high neopterin excretion levels. The other variables did not influence neopterin levels significantly. Our findings are in accord with recent data on the prevalence and mean titres of antibodies to the circumsporozoite protein of Plasmodium falciparum, which indicate that in endemic areas acquired humoral immunity develops slowly with increasing age, while prevalence and severity of disease decline.
在体外，新蝶呤是一种吡嗪并-[2,3-d]-嘧啶化合物，由人单核细胞-巨噬细胞在受到活化T淋巴细胞的上清液或重组γ干扰素诱导后产生。在体内，对尿液或血清中的新蝶呤进行测定，可为细胞介导的免疫反应的激活程度提供一种灵敏且特异的检测方法。对128名经寄生虫学证实患有疟疾的坦桑尼亚人（年龄在6个月至54岁之间）的尿新蝶呤水平进行了测量。将117名先前未接受治疗的患者亚组的水平与先前报道的来自泰国曼谷的19名未治疗疟疾患者（年龄在7至62岁之间）的水平进行了比较。分析了年龄、发热、寄生虫血症、症状持续时间和疟疾地方流行程度等伴随变量对新蝶呤排泄水平的影响。在泰国患者中，水平显著高于年龄相仿的坦桑尼亚受试者。在坦桑尼亚患者中，检测到年龄的压倒性影响，儿童的新蝶呤排泄水平极高。其他变量对新蝶呤水平没有显著影响。我们的研究结果与关于恶性疟原虫环子孢子蛋白抗体的流行率和平均滴度的最新数据一致，这些数据表明，在流行地区，获得性体液免疫随着年龄的增长发展缓慢，而疾病的流行率和严重程度则下降。
