一种带正电荷的聚乙二醇二丙烯酸酯水凝胶作为驱动性肌肉组织工程支架的表征与优化
Characterization and optimization of a positively charged poly (ethylene glycol)diacrylate hydrogel as an actuating muscle tissue engineering scaffold.
作者信息
Scott Tracy E, Khalili Amin, Newton Brandon, Warren Robert, Browe Daniel P, Freeman Joseph W
机构信息
Department of Biomedical Engineering, Rutgers, The State University of New Jersey, 599 Taylor Road, Piscataway, NJ 08854, USA.
出版信息
Polym Adv Technol. 2019 Oct;30(10):2604-2612. doi: 10.1002/pat.4681. Epub 2019 Jul 19.
Hydrogels have been used for many applications in tissue engineering and regenerative medicine due to their versatile material properties and similarities to the native extracellular matrix. Poly (ethylene glycol) diacrylate (PEGDA) is an ionic electroactive polymer (EAP), a material that responds to an electric field with a change in size or shape while in an ionic solution, that may be used in the development of hydrogels. In this study, we have investigated a positively charged EAP that can bend without the need of external ions. PEGDA was modified with the positively charged molecule 2-(methacryloyloxy)ethyl-trimethylammonium chloride (MAETAC) to provide its own positive ions. This hydrogel was then characterized and optimized for bending and cellular biocompatibility with C2C12 mouse myoblast cells. Studies show that the polymer responds to an electric field and supports C2C12 viability.
由于水凝胶具有多种材料特性且与天然细胞外基质相似，因此已在组织工程和再生医学中得到广泛应用。聚（乙二醇）二丙烯酸酯（PEGDA）是一种离子型电活性聚合物（EAP），这种材料在离子溶液中会因电场作用而发生尺寸或形状变化，可用于水凝胶的开发。在本研究中，我们研究了一种带正电荷的EAP，它无需外部离子就能弯曲。PEGDA用带正电荷的分子2-（甲基丙烯酰氧基）乙基三甲基氯化铵（MAETAC）进行了改性，以提供自身的正离子。然后对这种水凝胶进行了表征，并针对其与C2C12小鼠成肌细胞的弯曲性能和细胞生物相容性进行了优化。研究表明，该聚合物对电场有反应，并能支持C2C12细胞的存活。
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