Browe Daniel P, Wood Caroline, Sze Matthew T, White Kristopher A, Scott Tracy, Olabisi Ronke M, Freeman Joseph W
School of Engineering, Biomedical Engineering, Rutgers University, Piscataway, NJ 08854, USA.
School of Engineering, Biomedical Engineering, The College of New Jersey, Ewing Township, NJ 08168, USA.
Polymer (Guildf). 2017 May 19;117:331-341. doi: 10.1016/j.polymer.2017.04.044. Epub 2017 Apr 18.
Large volume deficiencies in skeletal muscle tissue fail to heal with conservative treatments, and improved treatment methods are needed. Tissue engineered scaffolds for skeletal muscle need to mimic the optimal environment for muscle development by providing the proper electric, mechanical, and chemical cues. Electroactive polymers, polymers that change in size or shape in response to an electric field, may be able to provide the optimal environment for muscle growth. In this study, an electroactive polymer made from poly(ethylene glycol) diacrylate (PEGDA) and acrylic acid (AA) is characterized and optimized for movement and biocompatibility. Hydrogel sample thickness, overall polymer concentration, and the ratio of PEGDA to AA were found to significantly impact the actuation response. C2C12 mouse myoblast cells attached and proliferated on hydrogel samples with various ratios of PEGDA to AA. Future experiments will produce hydrogel samples combined with aligned guidance cues in the form of electrospun fibers to provide a favorable environment for muscle development.
骨骼肌组织中的大量缺损无法通过保守治疗愈合，因此需要改进治疗方法。用于骨骼肌的组织工程支架需要通过提供适当的电、机械和化学信号来模拟肌肉发育的最佳环境。电活性聚合物，即响应电场而改变大小或形状的聚合物，可能能够为肌肉生长提供最佳环境。在本研究中，对一种由聚（乙二醇）二丙烯酸酯（PEGDA）和丙烯酸（AA）制成的电活性聚合物进行了表征，并针对运动和生物相容性进行了优化。发现水凝胶样品厚度、聚合物总浓度以及PEGDA与AA的比例对驱动响应有显著影响。不同PEGDA与AA比例的水凝胶样品上附着并增殖了C2C12小鼠成肌细胞。未来的实验将制备结合有静电纺丝纤维形式的排列引导信号的水凝胶样品，以为肌肉发育提供有利环境。
