Ihara Y, Taii S, Mori T
Department of Gynecology and Obstetrics, Kyoto University, Faculty of Medicine, Japan.
Endocrinol Jpn. 1987 Dec;34(6):887-96. doi: 10.1507/endocrj1954.34.887.
The expression of renin and angiotensinogen genes in the human placenta and related tissues has been examined by RNA blot hybridization analysis with specific human complementary DNA (cDNA) probes. Renin mRNA was detectable in the chorion throughout pregnancy and in the hydatidiform moles, but not in the decidua, amnion or myometrium. The relative concentration of renin mRNA in the chorion was at the highest level in early pregnancy and decreased thereafter, while the total amount contained in the whole placenta was at the lowest level in early pregnancy, and increased thereafter, reaching at term about one-sixth of the total renin mRNA in the kidney. Hydatidiform moles had an even higher concentration of renin mRNA than the early chorion. There was no significant difference in either the relative concentration or the total renin mRNA content in the placentae from 4 normal and 4 toxemic pregnancies. Angiotensinogen mRNA was undetectable in any of the placental tissues, hydatidiform moles or myometrium. These results show that renin is synthesized in the placenta, possibly to play some physiological role locally by utilizing angiotensinogen which is abundantly present in the maternal systemic circulation.
采用特异性人互补DNA（cDNA）探针，通过RNA印迹杂交分析检测了人胎盘及相关组织中肾素和血管紧张素原基因的表达。在整个孕期的绒毛膜以及葡萄胎中可检测到肾素mRNA，但在蜕膜、羊膜或子宫肌层中未检测到。绒毛膜中肾素mRNA的相对浓度在妊娠早期处于最高水平，之后下降，而整个胎盘中肾素mRNA的总量在妊娠早期处于最低水平，之后增加，足月时达到肾脏中肾素mRNA总量的约六分之一。葡萄胎中肾素mRNA的浓度甚至高于早期绒毛膜。4例正常妊娠和4例中毒妊娠胎盘的肾素mRNA相对浓度或总量均无显著差异。在任何胎盘组织、葡萄胎或子宫肌层中均未检测到血管紧张素原mRNA。这些结果表明，肾素在胎盘中合成，可能通过利用母体全身循环中大量存在的血管紧张素原在局部发挥某种生理作用。
