Department of Pharmacology, Institute of Psychiatry and Neurology, Warsaw, Poland.
Basic Clin Pharmacol Toxicol. 2021 Mar;128(3):419-429. doi: 10.1111/bcpt.13545. Epub 2020 Dec 18.
Hair is considered an efficient tool to investigate drug-related histories; thus, the selection of the method of sample preparation is important to obtain a reliable result. The aim of this study was to compare two methods of hair preparation (cutting and pulverizing) to analyse levetiracetam concentration in hair. An additional aim was to evaluate the potential usefulness of the levetiracetam concentration measured as an index of a dosing schedule. Four groups of 12 rats were included in the experiment. Depending on the group, the rats received 10 mg/kg of levetiracetam intraperitoneally every 24, 48 and 72 hours for 30 days. The control group was not treated. At the end of the drug administration, the rats' hair was shaved, cut or pulverized and analysed by the LC/MS-MS method to determine the concentration of levetiracetam. A stronger correlation between the mean hair levetiracetam concentration in hair and the number of drug doses was found in pulverized hair than in cut hair. A smaller standard deviation between the results was obtained in the case of pulverized hair. The results indicate that pulverization gives a more reliable result of drug concentration in hair than cutting and that drug concentration in hair can reflect the scheme of levetiracetam administration.
毛发被认为是研究药物相关历史的有效工具；因此，选择合适的样品制备方法对于获得可靠的结果非常重要。本研究旨在比较两种毛发制备方法（切割和粉碎），以分析毛发中左乙拉西坦的浓度。另一个目的是评估所测左乙拉西坦浓度作为给药方案指标的潜在用途。实验包括四组 12 只大鼠。根据组别不同，大鼠每 24、48 和 72 小时腹腔内给予 10mg/kg 的左乙拉西坦，持续 30 天。对照组未进行治疗。在药物给药结束时，剃光大鼠的毛发，然后将毛发切割或粉碎，并通过 LC/MS-MS 方法进行分析，以确定左乙拉西坦的浓度。与切割毛发相比，粉碎毛发的平均毛发左乙拉西坦浓度与药物剂量之间的相关性更强。在粉碎毛发的情况下，结果的标准差更小。结果表明，粉碎法比切割法更能可靠地反映毛发中的药物浓度，且毛发中的药物浓度可以反映左乙拉西坦的给药方案。
