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从乌鸦( rook )巢土壤中分离出角蛋白水解酶和 N、S 矿化形式的产生菌。

and Isolated from Soils in Rook () Colonies as Producers of Keratinolytic Enzymes and Mineral Forms of N and S.

机构信息

Department of Environmental Microbiology, Faculty of Agrobioengineering, University of Life Sciences in Lublin, Leszczyńskiego 7 Street, 20-069 Lublin, Poland.

State School of Higher Education in Chełm, Institute of Agricultural Science, Pocztowa 54 Street, 22-100 Chełm, Poland.

出版信息

Int J Environ Res Public Health. 2020 Dec 8;17(24):9162. doi: 10.3390/ijerph17249162.

Abstract

Keratinolytic fungi representing the genus that were isolated from the soils of a rook () colony were used as biological agents for the disposal of waste feathers. The aim of this study was to assess the abilities of and fungi with a varied inflow of keratin matter to biodegrade waste feathers. The evaluation was based on the determination of feather mass loss, the activity of keratinolytic enzymes, and the content of mineral N and S forms. It was found that the activity of protease released by the fungi contributed to an increase in the level of soluble proteins and peptides and the concentration of ammonium ions, as well as alkalization of the culture medium. Keratinase activity was significantly correlated with sulfate release, especially in cultures. The strains of fungi isolated from the soil with the highest supply of organic matter, i.e., strains III, IV, and V, had the lowest enzymatic activity, compared to the strains, but they released mineral nitrogen and sulfur forms that are highly important for fertilization, as well as nutritionally important peptides and amino acids. strains can be used for the management of waste feathers that can be applied in agricultural practice.

摘要

从一个(rook)群体的土壤中分离出的角蛋白分解真菌,属于,被用作处理废羽毛的生物制剂。本研究旨在评估具有不同角蛋白物质流入量的和真菌分解废羽毛的能力。评估基于羽毛质量损失、角蛋白分解酶活性以及矿质氮和硫形态的含量的测定。结果发现,真菌释放的蛋白酶活性促进了可溶性蛋白质和肽的水平以及铵离子浓度的增加,以及培养基的碱化。角蛋白酶活性与硫酸盐的释放呈显著相关,尤其是在培养物中。与分离自富含有机物土壤的菌株相比,III、IV 和 V 这三株真菌的酶活性最低,但它们释放的矿质氮和硫形态对施肥非常重要,以及营养上重要的肽和氨基酸。这些菌株可用于管理可应用于农业实践的废羽毛。

https://cdn.ncbi.nlm.nih.gov/pmc/blobs/4e36/7763491/4e4b938c206f/ijerph-17-09162-g001.jpg

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