Department of Rehabilitation Medicine, First Medical Center of Chinese PLA General Hospital, 28 Fuxing Road, Beijing, 100853, People's Republic of China.
BMC Musculoskelet Disord. 2020 Dec 10;21(1):831. doi: 10.1186/s12891-020-03793-2.
Platelet-rich plasma (PRP) is widely used to treat tendon injuries. Its therapeutic effect varies depending on the different cell components, and white blood cells (WBCs) may play an important role in this phenomenon. The purpose of this study was to evaluate how PRP with different concentrations of WBCs affect normal rabbit tendon and assess whether non-steroidal anti-inflammatory drugs (NSAIDs) can suppress the catabolic effects of WBCs.
Sixteen adult New Zealand White rabbits were used. Blood samples were collected from each rabbit, and PRP was extracted following two different protocols to obtain leukocyte-poor PRP (LP-PRP) and leukocyte-rich PRP (LR-PRP). LP-PRP or LR-PRP was injected into the patellar tendon of each rabbit, while normal saline (NS) was injected as control. In LR-PRP + NSAID group, Parecoxib was administered after LR-PRP injection. For each group, 2 rabbits were euthanatized at day 5 and 14. The patellar tendons were collected and stained with hematoxylin and eosin. A semi-quantitative approach was used to assess the inflammatory response and tendon destruction based on the evaluation of the WBCs, vascularization, fiber structure, and fibrosis.
The LR-PRP group exhibited a higher total tendon score than the LP-PRP group at day 5 after PRP injection, but there was no significant difference between the two groups at day 14. For the NSAID group, the tendon score was lower than that of the LR-PRP group both at day 5 and 14.
LR-PRP can promote a higher inflammatory response than LP-PRP in the normal rabbit patellar tendon, and this effect can be suppressed by NSAIDs.
富含血小板的血浆（PRP）被广泛用于治疗肌腱损伤。其治疗效果取决于不同的细胞成分，白细胞（WBC）可能在这一现象中发挥重要作用。本研究旨在评估不同 WBC 浓度的 PRP 如何影响正常兔肌腱，并评估非甾体抗炎药（NSAIDs）是否能抑制 WBC 的分解代谢作用。
本研究使用了 16 只成年新西兰白兔。从每只兔子身上采集血液样本，采用两种不同的方案提取 PRP，以获得白细胞较少的 PRP（LP-PRP）和白细胞丰富的 PRP（LR-PRP）。将 LP-PRP 或 LR-PRP 注入每只兔子的髌腱，而生理盐水（NS）则作为对照进行注射。在 LR-PRP+NSAID 组中，LR-PRP 注射后给予帕瑞昔布。对于每组，在第 5 天和第 14 天分别处死 2 只兔子。收集髌腱并进行苏木精和伊红染色。采用半定量方法，根据 WBC、血管化、纤维结构和纤维化的评估，评估炎症反应和肌腱破坏程度。
在 PRP 注射后第 5 天，LR-PRP 组的总肌腱评分高于 LP-PRP 组，但第 14 天两组间无显著差异。对于 NSAID 组，第 5 天和第 14 天的肌腱评分均低于 LR-PRP 组。
LR-PRP 可在正常兔髌腱中引起比 LP-PRP 更高的炎症反应，而 NSAIDs 可抑制这种反应。
