Soltani K, Choy R K, Lorincz A L
J Invest Dermatol. 1977 Nov;69(5):439-41. doi: 10.1111/1523-1747.ep12510730.
Specific antitreponemal antibodies have been demonstrated by immunofluorescence techniques in the lymphoplasmocytic infiltrates which characterize early symphilitic lesions. A purified suspension of Nichols strain Treponema pallidum was sonified and labeled with fluorescein isothiocyanate and applied to cryostat sections of 12 biopsy specimens from the cutaneous lesions of 11 patients with proven secondary syphilis, using a modified direct immunofluorescence procedure. Specimens from various inflammatory dermatoses processed similarly served as controls. Granular fluorescence was noted in the dermis in 9 of the 12 specimens corresponding to areas of heavy plasma cell infiltration and some fluorescence was found directly on plasma cells which were identified by subsequent hematoxylin and eosin staining. This fluorescence could be blocked by prior incubation of the sections with unlabeled sonified treponemal suspension. Control slides did not reveal any fluorescence. The use of labeled treponemal antigen may aid the tissue diagnosis of early syphilitic lesions which can mimic a variety of dermatological disorders.
通过免疫荧光技术已在具有早期梅毒病变特征的淋巴浆细胞浸润中证实了特异性抗梅毒螺旋体抗体。将Nichols株梅毒螺旋体的纯化悬液超声处理并用异硫氰酸荧光素标记，采用改良的直接免疫荧光法，将其应用于11例确诊为二期梅毒患者皮肤病变的12份活检标本的冰冻切片。同样处理的各种炎症性皮肤病标本用作对照。在12份标本中的9份标本的真皮中观察到颗粒状荧光，对应于浆细胞大量浸润的区域，并且在随后经苏木精和伊红染色鉴定的浆细胞上直接发现了一些荧光。这种荧光可以通过用未标记的超声处理的梅毒螺旋体悬液预先孵育切片来阻断。对照玻片未显示任何荧光。使用标记的梅毒螺旋体抗原可能有助于早期梅毒病变的组织诊断，这些病变可能模仿多种皮肤病。
