Soltani K, Aronson I K, Brickman F, Lorincz A L
J Infect Dis. 1978 Aug;138(2):222-6. doi: 10.1093/infdis/138.2.222.
By direct immunofluorescence, with use of fluorescein-labeled sonified Treponema pallidum, specific antibodies were detected in the tissue infiltrates of cutaneous syphilomas in rabbits. Specimens from cutaneous and mucosal inflammatory lesions induced by intradermal injection of a keratinous substance from an epidermal cyst served as controls. Granular fluorescence was detected in the dermis of 11 of the 12 syphiloma specimens and corresponded to areas of heavy plasma cell infiltrates, and some fluorescence was found directly on plasma cells identified by subsequent staining with hematoxylin and eosin. This fluorescence could be blocked by preabsorption. Control slides did not show any fluorescence. Immunopathologic techniques using labeled antigens may be of diagnostic value in syphilis and other infectious disorders which feature specific infiltrates.
通过直接免疫荧光法，使用荧光素标记的超声处理梅毒螺旋体，在兔皮肤梅毒瘤的组织浸润中检测到特异性抗体。将来自表皮囊肿的角质物质皮内注射诱导的皮肤和粘膜炎症病变标本作为对照。在12个梅毒瘤标本中的11个标本的真皮中检测到颗粒状荧光，其与大量浆细胞浸润区域相对应，并且通过随后用苏木精和伊红染色鉴定，在浆细胞上直接发现了一些荧光。这种荧光可通过预吸收而被阻断。对照玻片未显示任何荧光。使用标记抗原的免疫病理技术在梅毒和其他具有特异性浸润特征的感染性疾病中可能具有诊断价值。
