用于检测人类梅毒特异性IgM抗体的微酶联免疫吸附测定法。
Microenzyme-linked immunosorbent assay for the detection of specific IgM antibodies in human syphilis.
作者信息
Lindenschmidt E G, Laufs R, Müller F
出版信息
Br J Vener Dis. 1983 Jun;59(3):151-6. doi: 10.1136/sti.59.3.151.
Abstract
An enzyme-linked immunosorbent assay (ELISA) using a highly purified soluble antigen prepared from pathogenic Treponema pallidum (Nichols) was carried out to detect specific IgM antibodies in syphilis. Serum specimens from 365 patients with untreated or treated syphilis were tested by the ELISA and by the 19S(IgM) fluorescent treponemal antibody-absorbed test. In the ELISA sera were preabsorbed with aggregated human IgG to eliminate any rheumatoid factor present. Sera from 331 healthy blood donors served as controls. The results of both tests correlated well. The specificity and sensitivity of the treponemal ELISA were both greater than 97%. The assay was easy to perform and may be automated.
摘要
采用从致病性梅毒螺旋体（Nichols株）制备的高度纯化可溶性抗原进行酶联免疫吸附试验（ELISA），以检测梅毒特异性IgM抗体。用ELISA和19S（IgM）荧光密螺旋体抗体吸收试验检测了365例未经治疗或已治疗梅毒患者的血清标本。在ELISA中，血清先用聚集的人IgG预吸收，以消除任何存在的类风湿因子。331名健康献血者的血清作为对照。两种试验结果相关性良好。密螺旋体ELISA的特异性和敏感性均大于97%。该检测方法易于操作，且可实现自动化。
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本文引用的文献
1
Protein measurement with the Folin phenol reagent.使用福林酚试剂进行蛋白质测定。
J Biol Chem. 1951 Nov;193(1):265-75.
2
3
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4
5
Demonstration of specific 19S(IgM) antibodies in untreated and treated syphilis. Comparative studies of the 19S(IgM)-FTA test, the 19S(IgM)-TPHA test, and the solid phase haemadsorption assay.未经治疗和已治疗梅毒中特异性19S（IgM）抗体的检测。19S（IgM）-FTA试验、19S（IgM）-TPHA试验及固相血细胞吸附试验的比较研究。
Br J Vener Dis. 1982 Feb;58(1):12-7. doi: 10.1136/sti.58.1.12.
6
[Efficiency and limitations of the IgM-FTA-ABS-test in the routine serodiagnostic of syphilis (author's transl)].
Zentralbl Bakteriol A. 1981 Mar;249(1):104-23.
7
A highly sensitive periodic acid-silver stain for 1,2-diol groups of glycoproteins and polysaccharides in polyacrylamide gels.一种用于聚丙烯酰胺凝胶中糖蛋白和多糖的1,2 -二醇基团的高灵敏度高碘酸 - 银染色法。
Anal Biochem. 1982 Jan 15;119(2):325-9. doi: 10.1016/0003-2697(82)90593-0.
8
Immunoglobulin M antibodies to hepatitis B core antigen: evaluation of enzyme immunoassay for diagnosis of hepatitis B virus infection.乙型肝炎核心抗原的免疫球蛋白M抗体：酶免疫测定法在诊断乙型肝炎病毒感染中的评估
J Clin Microbiol. 1981 Apr;13(4):618-26. doi: 10.1128/jcm.13.4.618-626.1981.
9
Some characteristics of human immunoconglutinin.人免疫胶固素的一些特性。
J Immunol. 1966 Apr;96(4):637-45.
10
Cleavage of structural proteins during the assembly of the head of bacteriophage T4.在噬菌体T4头部组装过程中结构蛋白的切割
Nature. 1970 Aug 15;227(5259):680-5. doi: 10.1038/227680a0.
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