Aarti Chirom, Khusro Ameer, Agastian Paul, Darwish Noura M, Al Farraj Dunia A
Research Department of Plant Biology and Biotechnology, Loyola College (Affiliated to University of Madras), Nungambakkam, Chennai 600034, Tamil Nadu, India.
Faculty of Science, Biochemistry Department, Ain Shams University, Cairo, Egypt.
Saudi J Biol Sci. 2020 Dec;27(12):3235-3248. doi: 10.1016/j.sjbs.2020.09.049. Epub 2020 Sep 29.
Soil is an integral part of ecosystem which is niche for varieties of microflora. The present study was investigated to isolate varied strains of bacteria from soil samples of three different geographical regions of Tamil Nadu (India) and evaluate their hydrolytic enzymes (amylase, cellulase, and inulinase) producing potentialities. Among 72 bacterial cultures isolated from Ambattur Industrial Estate, Neyveli Lignite Corporation, and Arignar Anna Zoological Park regions, 41.66, 38.88, and 36.11% of isolates were observed amylase, cellulase, and inulinase producers, respectively. On the other hand, 20.83% of total bacteria isolated from all three regions exhibited concurrent production of amylase, cellulase, and inulinase. Potent isolates depicting maximum enzyme activities were identified as strain ALA1, strain ALA3, strain ALA4, and strain ALA5 based on molecular characterization tools. Further, the thermodynamics parameters, open reading frames (ORFs) regions, and guanine-cytosine (GC) content were determined by distinct bioinformatics tools using 16S rRNA sequences of strains. Minimum free energy values for strain ALA1, strain ALA3, strain ALA4, and strain ALA5 were calculated as -480.73, 478.76, -496.63, and -479.03 kcal/mol, respectively. Mountain plot and entropy predicted the hierarchical representation of RNA secondary structure. The GC content of sequence for strain ALA1, strain ALA3, strain ALA4, and strain ALA5 was calculated as 53.06, 52.94, 56.78, and 53.06%, respectively. Nine ORFs were obtained for strain ALA1, strain ALA3, and strain ALA5 while 10 ORFs were observed for strain ALA4. Additionally, bootstrap tree demonstrated close resemblance of strains with existing bacteria of similar genus. Findings showed higher variability of bacterial diversity as hydrolytic enzymes producers in the investigated geographical regions.
土壤是生态系统的重要组成部分，是各种微生物群落的栖息地。本研究旨在从印度泰米尔纳德邦三个不同地理区域的土壤样本中分离出不同的细菌菌株，并评估它们产生水解酶（淀粉酶、纤维素酶和菊粉酶）的潜力。从安巴图尔工业园区、尼韦利褐煤公司和阿里亚纳·安娜动物园地区分离出的72种细菌培养物中，分别有41.66%、38.88%和36.11%的分离物被观察到能产生淀粉酶、纤维素酶和菊粉酶。另一方面，从所有三个地区分离出的细菌中，有20.83%的细菌同时产生淀粉酶、纤维素酶和菊粉酶。根据分子表征工具，将表现出最大酶活性的强效分离物鉴定为ALA1菌株、ALA3菌株、ALA4菌株和ALA5菌株。此外，使用菌株的16S rRNA序列，通过不同的生物信息学工具确定了热力学参数、开放阅读框（ORF）区域和鸟嘌呤-胞嘧啶（GC）含量。ALA1菌株、ALA3菌株、ALA4菌株和ALA5菌株的最小自由能值分别计算为-480.73、-478.76、-496.63和-479.03千卡/摩尔。山形图和熵预测了RNA二级结构的层次表示。ALA1菌株、ALA3菌株、ALA4菌株和ALA5菌株序列的GC含量分别计算为53.06%、52.94%、56.7
