Nagai Takashi
Institute for Agro-Environmental Sciences, NARO.
J Pestic Sci. 2020 Nov 20;45(4):223-229. doi: 10.1584/jpestics.D20-035.
Five species of aquatic fungi and fungus-like organisms were used for toxicity assays with seven fungicides to determine the differences in species sensitivity. A microplate toxicity assay with adenosine triphosphate luminescence detection was used as an efficient and economical high-throughput assay. The obtained toxicity data were standardized based on the species sensitivity distribution method. Species sensitivity differed among the fungicides: was most sensitive to hydroxyisoxazole, isoprothiolane, and ferimzone; was most sensitive to tricyclazole; was most sensitive to ipconazole; was most sensitive to orysastrobin and kasugamycin. was not the most sensitive species to any of the tested fungicides. The ranges of ECs to fungal species were lower than to other aquatic organisms (primary producers, invertebrates, and vertebrates) for hydroxyisoxazole, kasugamycin, isoprothiolane, ipconazole, and ferimzone. These results suggest the usefulness of a battery of fungal species to assess the ecological effects of fungicides.
使用五种水生真菌和类真菌生物对七种杀菌剂进行毒性测定，以确定物种敏感性的差异。采用基于三磷酸腺苷发光检测的微孔板毒性测定法作为一种高效且经济的高通量测定法。所获得的毒性数据基于物种敏感性分布方法进行标准化。杀菌剂之间的物种敏感性存在差异： 对羟基异恶唑、异稻瘟净和嗪氨灵最为敏感； 对三环唑最为敏感； 对抑霉唑最为敏感； 对肟菌酯和春雷霉素最为敏感。 对任何一种测试杀菌剂都不是最敏感的物种。对于羟基异恶唑、春雷霉素、异稻瘟净、抑霉唑和嗪氨灵，真菌物种的半数有效浓度（EC）范围低于其他水生生物（初级生产者、无脊椎动物和脊椎动物）。这些结果表明，一系列真菌物种对于评估杀菌剂的生态效应是有用的。
