Nagai Takashi
Institute for Agro-Environmental Sciences, NARO, 3-1-3 Kannondai, Tsukuba, Ibaraki 305-8604, Japan.
J Pestic Sci. 2019 Nov 20;44(4):225-232. doi: 10.1584/jpestics.D19-039.
Seven algal species were used to conduct toxicity assays with 12 herbicides to determine differences in species sensitivity. A fluorescence microplate toxicity assay was used as an efficient and economical high-throughput assay. The obtained toxicity data were standardized based on the species sensitivity distribution concept. The most-sensitive individual species differed among herbicides: was most sensitive to chloronitrofen and pendimethalin; was most sensitive to chlorpropham; was most sensitive to diquat, glyphosate, and dichlobenil; was most sensitive to trifluralin; and was most sensitive to glufosinate, asulam, and 2,4-D. Surprisingly, (formerly ) , a standard green alga, was not the most sensitive to any of the herbicides. The results clearly showed that a single algal species cannot represent the algal assemblage in terms of sensitivity. Therefore, multispecies algal toxicity data sets are essential for assessing the ecological effect of herbicides.
使用7种藻类物种对12种除草剂进行毒性测定，以确定物种敏感性差异。荧光微孔板毒性测定法被用作一种高效且经济的高通量测定方法。所获得的毒性数据基于物种敏感性分布概念进行了标准化。对不同除草剂最敏感的单个物种有所不同： 对氯硝酚和二甲戊灵最敏感； 对氯苯胺灵最敏感； 对敌草快、草甘膦和二氯苯腈最敏感； 对氟乐灵最敏感； 对草铵膦、杀草强和2,4 - D最敏感。令人惊讶的是，标准绿藻 （以前称为 ）对任何一种除草剂都不是最敏感的。结果清楚地表明，就敏感性而言，单一藻类物种不能代表藻类群落。因此，多物种藻类毒性数据集对于评估除草剂的生态效应至关重要。
