Medeiros Isaiane, de Oliveira Grazielle Louise Ribeiro, de Queiroz Jaluza Luana Carvalho, de Carvalho Gomes Camila, de Carvalho Fabiana Maria Coimbra, de Souza Lima Maíra Conceição Jerônimo, Serquiz Alexandre Coelho, de Andrade Santos Pedro Paulo, da Silva Camillo Christina, Maciel Bruna Leal Lima, de Araújo Morais Ana Heloneida, Passos Thaís Souza
Postgraduate Program in Nutrition, Health Sciences Center, Federal University of Rio Grande do Norte, Natal, Brazil.
Postgraduate Program in Biochemistry, Center for Biosciences, Federal University of Rio Grande do Norte, Natal, Brazil.
Biotechnol Rep (Amst). 2020 Nov 21;28:e00567. doi: 10.1016/j.btre.2020.e00567. eCollection 2020 Dec.
The safety and bioactive potential of crude carotenoid extract from Cantaloupe melon nanoencapsulated in porcine gelatin (EPG) were evaluated in a chronic inflammatory experimental model. Animals were fed a high glycemic index and high glycemic load (HGLI) diet for 17 weeks and treated for ten days with 1) HGLI diet, 2) standard diet, 3) HGLI diet + crude carotenoid extract (CE) (12.5 mg/kg), and 4) HGLI diet + EPG (50 mg/kg). General toxicity signals were investigated, considering body weight, food intake, hematological, biochemical parameters, relative weight, morphology, and histopathology of organs. The biochemical parameters indicated the low toxicity of EPG. Acute hepatitis was observed in animals' livers, but CE and EPG groups presented improved tissue appearance. Chronic enteritis was observed in animals, with villi and intestinal glands preservation in the EPG group. The results suggest the safety and the bioactive effect of EPG, possibly related to its anti-inflammatory potential.
在慢性炎症实验模型中评估了包裹于猪明胶（EPG）中的哈密瓜粗类胡萝卜素提取物的安全性和生物活性潜力。动物被给予高血糖指数和高血糖负荷（HGLI）饮食17周，并在十天内接受以下处理：1）HGLI饮食，2）标准饮食，3）HGLI饮食 + 粗类胡萝卜素提取物（CE）（12.5毫克/千克），以及4）HGLI饮食 + EPG（50毫克/千克）。研究了一般毒性信号，考虑了体重、食物摄入量、血液学、生化参数、相对重量、器官形态和组织病理学。生化参数表明EPG的毒性较低。在动物肝脏中观察到急性肝炎，但CE组和EPG组的组织外观有所改善。在动物中观察到慢性肠炎，EPG组的绒毛和肠腺得以保留。结果表明EPG具有安全性和生物活性作用，这可能与其抗炎潜力有关。
