Postgraduate Program in Nutrition, Health Sciences Center, Federal University of Rio Grande do Norte, Natal, RN 59078-970, Brazil.
Postgraduate Program in Biochemistry, Center for Biosciences, Federal University of Rio Grande do Norte, Natal, RN 59078-970, Brazil.
Food Chem. 2021 Jun 30;348:129055. doi: 10.1016/j.foodchem.2021.129055. Epub 2021 Jan 14.
The study evaluated the potential and antioxidant stability of nanoencapsulated carotenoid-rich extract (CE) from Cantaloupe melon (EPG). DPPH and ABTS radical scavenging assays were used to investigate the nanoencapsulation effect on antioxidant potential. CE and EPG stability were evaluated at 25 °C and 5 °C, with and without light (1600 lx) for 60 days, determining the β-carotene concentration by UHPLC and antioxidant potential by ABTS. The antioxidant potential of carotenoids increased after nanoencapsulation (57-59%). After 60 days, there was low retention of β-carotene (0-43.6%) in the CE, mainly at 25 °C light (0.00%) and dark (10.0%), and total loss of activity in the four conditions. EPG preserved the β-carotene concentration in the dark at 25 °C (99.0%) and in the light (83.1%) and dark (99.0%) at 5 °C, maintaining the antioxidant potential (68.7-48.3%). Therefore, EPG enhanced and stabilized the antioxidant potential of carotenoids, beneficial to human health.
该研究评估了来自哈密瓜的富含类胡萝卜素的浓缩提取物（CE）的纳米封装的潜力和抗氧化稳定性（EPG）。DPPH 和 ABTS 自由基清除试验用于研究纳米封装对抗氧化潜力的影响。在 25°C 和 5°C 下，分别在有光（1600 lx）和无光条件下（5°C 和 25°C，无光照），对 CE 和 EPG 稳定性进行为期 60 天的评估，通过 UHPLC 测定 β-胡萝卜素浓度，通过 ABTS 测定抗氧化潜力。类胡萝卜素的抗氧化潜力在纳米封装后增加（57-59%）。60 天后，CE 中的 β-胡萝卜素保留率较低（0-43.6%），主要在 25°C 光照（0.00%）和黑暗（10.0%）条件下，在四种条件下活性完全丧失。EPG 在 25°C 黑暗条件下（99.0%）和 5°C 光照（83.1%）和黑暗（99.0%）条件下保留了 β-胡萝卜素浓度，保持了抗氧化潜力（68.7-48.3%）。因此，EPG 增强并稳定了类胡萝卜素的抗氧化潜力，对人类健康有益。
