Chen Angela, Majdinasab Elleana J, Fiori Mariana C, Liang Hongjun, Altenberg Guillermo A
School of Medicine, Texas Tech University Health Sciences Center, Lubbock, TX, United States.
Department of Cell Physiology and Molecular Biophysics, and Center for Membrane Protein Research, School of Medicine, Texas Tech University Health Sciences Center, Lubbock, TX, United States.
Front Bioeng Biotechnol. 2020 Nov 16;8:598450. doi: 10.3389/fbioe.2020.598450. eCollection 2020.
Membrane proteins (MPs) are essential to many organisms' major functions. They are notorious for being difficult to isolate and study, and mimicking native conditions for studies has proved to be a challenge. Lipid nanodiscs are among the most promising platforms for MP reconstitution, but they contain a relatively labile lipid bilayer and their use requires previous protein solubilization in detergent. These limitations have led to the testing of copolymers in new types of nanodisc platforms. Polymer-encased nanodiscs and polymer nanodiscs support functional MPs and address some of the limitations present in other MP reconstitution platforms. In this review, we provide a summary of recent developments in the use of polymers in nanodiscs.
膜蛋白（MPs）对于许多生物体的主要功能至关重要。它们因难以分离和研究而声名狼藉，并且事实证明，模拟天然条件进行研究是一项挑战。脂质纳米盘是用于膜蛋白重构最有前景的平台之一，但它们包含相对不稳定的脂质双层，并且其使用需要蛋白质事先在去污剂中溶解。这些局限性促使人们在新型纳米盘平台中测试共聚物。聚合物包裹的纳米盘和聚合物纳米盘能够支持功能性膜蛋白，并解决了其他膜蛋白重构平台中存在的一些局限性。在本综述中，我们总结了聚合物在纳米盘中应用的最新进展。
