Kim Hyeon Joong, Lee Jae Hoon, Ahn Dong Uk, Paik Hyun-Dong
Department of Food Science and Biotechnology of Animal Resources, Konkuk University, Seoul 05029, Korea.
Department of Animal Science, Iowa State University, Ames, IA 50011, USA.
Food Sci Anim Resour. 2020 Nov;40(6):1001-1013. doi: 10.5851/kosfa.2020.e71. Epub 2020 Nov 1.
The formation of biofilms on the enamel surface of teeth by is an important step in dental plaque formation, demineralization, and early caries because the biofilm is where other bacteria involved in dental caries attach, grow, and proliferate. The objectives of this study were to determine the effect of phosvitin (PSV) on the biofilm formation, exopolysaccharides (EPS) production, adherence activity of , and the expression of genes related to the compounds essential for biofilm formation (quorum-sensing inducers and components of biofilm matrix) by . PSV significantly reduced the biofilm-forming activity of and increased the degradation of preformed biofilms by . PSV inhibited the adherence activity of by 31.9%-33.6%, and the production of EPS by 62%-65% depending upon the strains and the amount of PSV added. The expressions of genes regulating the production of EPS and the quorum-sensing-inducers (, and ) in all strains were down-regulated by PSV, but was down-regulated only in KCTC 5316. Therefore, the anti-biofilm-forming activity of PSV was accomplished through the inhibition of biofilm formation, adherence activity, and the production of quorum-sensing inducers and EPS by .
细菌在牙齿釉质表面形成生物膜是牙菌斑形成、脱矿和早期龋齿过程中的重要步骤，因为生物膜是其他参与龋齿的细菌附着、生长和增殖的场所。本研究的目的是确定磷蛋白（PSV）对细菌生物膜形成、胞外多糖（EPS）产生、细菌黏附活性以及细菌中与生物膜形成所需化合物（群体感应诱导物和生物膜基质成分）相关基因表达的影响。PSV显著降低了细菌的生物膜形成活性，并增加了其对预先形成的生物膜的降解。PSV抑制细菌的黏附活性达31.9%-33.6%，并根据菌株和添加的PSV量抑制EPS产生达62%-65%。PSV下调了所有细菌菌株中调节EPS产生和群体感应诱导物（、和）的基因表达，但仅在KCTC 5316中下调了。因此，PSV的抗生物膜形成活性是通过抑制细菌的生物膜形成、黏附活性以及群体感应诱导物和EPS的产生来实现的。
