Department of Food Science and Biotechnology of Animal Resource Konkuk University, Seoul 05029, Republic of Korea.
J Microbiol Biotechnol. 2022 Jan 28;32(1):56-63. doi: 10.4014/jmb.2109.09045.
This study aims to determine the antibiofilm effect of cell-free supernatant (CFS) of strains against strains. To study the antibiofilm mechanism against strains, antibacterial effects, cell surface properties (auto-aggregation and cell surface hydrophobicity), exopolysaccharide (EPS) production, and morphological changes were examined. The antibiofilm effect of KCCM 202399 CFS as morphological changes were evaluated by scanning electron microscopy (SEM) and confocal laser scanning microscopy (CLSM), compared with the control treatment. Among the strains, KCCM 202399 showed the highest antibiofilm effect on KCTC 5458. The antibacterial effect of KCCM 202399 against KCTC 5458 was investigated using the deferred method (16.00 mm). The minimum inhibitory concentration of KCCM 202399 against KCTC 5458 was 25.00%. Compared with the control treatment, KCCM 202399 CFS inhibited the bacterial adhesion of KCTC 5458 by decreasing auto-aggregation, cell surface hydrophobicity, and EPS production (45.91%, 40.51%, and 67.44%, respectively). KCCM 202399 CFS inhibited and eradicated the KCTC 5458 biofilm. Therefore, these results suggest that KCCM 202399 CFS may be used to develop oral health in the probiotic industry.
本研究旨在确定菌株的无细胞上清液（CFS）对菌株的抗生物膜效应。为了研究针对菌株的抗生物膜机制，检测了抗菌作用、细胞表面特性（自聚集和细胞表面疏水性）、胞外多糖（EPS）产生和形态变化。通过扫描电子显微镜（SEM）和共聚焦激光扫描显微镜（CLSM）评估了菌株 KCCM 202399 CFS 的抗生物膜作用，与对照处理进行了比较。在所测试的菌株中，菌株 KCCM 202399 对 KCTC 5458 表现出最高的抗生物膜作用。使用延迟法（16.00mm）研究了菌株 KCCM 202399 对 KCTC 5458 的抗菌作用。菌株 KCCM 202399 对 KCTC 5458 的最小抑菌浓度为 25.00%。与对照处理相比，菌株 KCCM 202399 CFS 通过降低自聚集、细胞表面疏水性和 EPS 产生来抑制 KCTC 5458 的细菌黏附（分别为 45.91%、40.51%和 67.44%）。菌株 KCCM 202399 CFS 抑制并消除了 KCTC 5458 生物膜。因此，这些结果表明，菌株 KCCM 202399 CFS 可能被用于益生菌产业中的口腔健康开发。
