Department of Food Science and Biotechnology of Animal Resources, Konkuk University, Seoul 05029, Republic of Korea.
J Microbiol Biotechnol. 2024 Jan 28;34(1):116-122. doi: 10.4014/jmb.2306.06001. Epub 2023 Sep 8.
Dental caries has known as an infectious disease that is considered a serious global public health problem. Recently, report indicate that probiotics play a vital role in maintaining oral health. Therefore, this study aimed to evaluate the prevention effects of Ln4 against dental infection by the pathogenic bacterium KCTC 5124 through biofilm formation inhibition. To evaluate such prevention effects against KCTC 5124, antimicrobial activity, auto-aggregation, co-aggregation, cell surface hydrophobicity, total exopolysaccharide (EPS) production rate, and biofilm formation were analyzed. Results showed that Ln4 showed higher antimicrobial activity than GG (LGG). In the group treated with Ln4, the co-aggregation (58.85%), cell surface hydrophobicity (16.75%), and EPS production rate (73.29%) values were lower than those of LGG and the negative control. Additionally, crystal violet staining and confocal laser scanning microscopy (CLSM) revealed that Ln4 effectively inhibited biofilm formation in KCTC 5124. Therefore, Ln4 could be used in the industry as a probiotics to prevent and improve oral health.
龋齿是一种传染病，被认为是一个严重的全球公共卫生问题。最近，有报道称益生菌在维持口腔健康方面起着至关重要的作用。因此，本研究旨在通过抑制生物膜形成来评估 Ln4 对致病菌 KCTC 5124 引起的口腔感染的预防作用。为了评估 Ln4 对 KCTC 5124 的这种预防作用，分析了其抗菌活性、自聚集、共聚、细胞表面疏水性、总胞外多糖（EPS）产生率和生物膜形成。结果表明，Ln4 比 GG（LGG）具有更高的抗菌活性。在 Ln4 处理组中，共聚（58.85%）、细胞表面疏水性（16.75%）和 EPS 产生率（73.29%）的值低于 LGG 和阴性对照组。此外，结晶紫染色和共聚焦激光扫描显微镜（CLSM）显示，Ln4 能有效抑制 KCTC 5124 的生物膜形成。因此，Ln4 可以在工业中用作益生菌来预防和改善口腔健康。
