Rodríguez-Fernández Pablo, Gómez Andromeda-Celeste, Gibert Isidre, Prat-Aymerich Cristina, Domínguez Jose
Servei de Microbiologia, Hospital Universitari Germans Trias i Pujol, Institut d'Investigació en Ciències de la Salut Germans Trias i Pujol, Badalona, Spain.
CIBER Enfermedades Respiratorias (CIBERES), Madrid, Spain.
Exp Lung Res. 2021 Feb-Mar;47(2):87-97. doi: 10.1080/01902148.2020.1854371. Epub 2020 Dec 11.
Smoking is a cause behind many diseases, including tuberculosis, and it is a risk factor for tuberculosis infection and mortality. Moreover, smoking is associated with a poor tuberculosis treatment outcome.
In this study, we focus on the effects of cigarette smoke on an infected cell culture treated with anti-tuberculosis drugs.
Cytotoxicity on THP-1, J774A.1 and MH-S cell lines and growth of exposed to a reference or a commercial cigarette was evaluated. THP-1 cell line was exposed to cigarette smoke, infected with and treated with anti-tuberculosis drugs. Apoptosis and death cell were also tested on BCG infected cells. Minimal inhibitory concentrations of anti-tuberculosis drugs were analyzed.
All cells lines showed viability values higher than 80% when exposed to cigarette smoke extract. However, THP-1 cell line infected with BCG and exposed to Marlboro cigarette smoke showed up to a 54% reduction of apoptotic cells than cells unexposed to smoke. exposed to Marlboro cigarette smoke for 11 days had an optical density 16% lower than unexposed bacteria. When cells were infected with , the intracellular recovery of CFUs showed up to a 0.66 log reduction in cells exposed to cigarette smoke extract because of a potential impairment in the phagocytosis. Macrophages treated with drugs showed up to a 2.55 log reduction in the intracellular load burden compared with non-treated ones. Despite poor treatment outcome on TB smoker patients, minimal inhibitory concentration of rifampicin increased only 2-fold in exposed to cigarette smoke.
Smoking interferes with tuberculosis treatment impairing the immunity of the host.
吸烟是包括肺结核在内的多种疾病的病因，是结核感染和死亡的危险因素。此外，吸烟与肺结核治疗效果不佳有关。
在本研究中，我们聚焦香烟烟雾对用抗结核药物治疗的感染细胞培养物的影响。
评估了参考香烟或市售香烟对THP-1、J774A.1和MH-S细胞系的细胞毒性以及细胞生长情况。将THP-1细胞系暴露于香烟烟雾中，用卡介苗感染并给予抗结核药物治疗。还对卡介苗感染的细胞进行了凋亡和死亡细胞检测。分析了抗结核药物的最低抑菌浓度。
所有细胞系暴露于香烟烟雾提取物时活力值均高于80%。然而，感染卡介苗并暴露于万宝路香烟烟雾中的THP-1细胞系，其凋亡细胞比未暴露于烟雾的细胞减少了54%。暴露于万宝路香烟烟雾11天的细菌光密度比未暴露的细菌低16%。当细胞用卡介苗感染时，由于吞噬作用可能受损，暴露于香烟烟雾提取物的细胞中集落形成单位的细胞内回收率显示最多降低0.66个对数。与未治疗的巨噬细胞相比，用药物治疗的巨噬细胞细胞内负荷负担最多降低2.55个对数。尽管肺结核吸烟者患者治疗效果不佳，但暴露于香烟烟雾中的结核杆菌对利福平的最低抑菌浓度仅增加了2倍。
吸烟会干扰肺结核治疗，损害宿主免疫力。
