Servei de Microbiologia, Hospital Universitari Germans Trias i Pujol, Institut d'Investigació en Ciències de la Salut Germans Trias i Pujol, CIBER Enfermedades Respiratorias (CIBERES), Badalona, Catalonia, Spain.
Institut de Biotecnologia i de Biomedicina (IBB), Universitat Autònoma de Barcelona (UAB), Bellaterra (Cerdanyola del Vallès), Barcelona, Catalonia, Spain.
PLoS One. 2020 Feb 10;15(2):e0228919. doi: 10.1371/journal.pone.0228919. eCollection 2020.
Cigarette smoking and tuberculosis are a significant cause of death worldwide. Several epidemiological studies have demonstrated cigarette smoking is a risk factor for tuberculosis. Electronic cigarettes have recently appeared as a healthier alternative to conventional smoking, although their impact in tuberculosis is not well understood. The aim of this study was to explore the effect of electronic cigarettes in phagocytosis of Mycobacterium tuberculosis and cytokines production. In vitro infection was carried out by exposing THP-1 macrophages to four electronic vapor extracts and the intracellular burden of M. tuberculosis was determined. The percentage of infection was evaluated by confocal microscopy and the cytokine production by Luminex. A reduction of intracellular M. tuberculosis burden in THP-1 macrophages was found after its exposure to electronic vapor extract; the same trend was observed by confocal microscopy when Mycobacterium bovis BCG-GFP strain was used. Electronic cigarettes stimulate a pro-inflammatory cytokine response. We conclude that electronic cigarettes impair the phagocytic function and the cytokine response to M. tuberculosis.
吸烟和结核病是全球范围内的重要死因。多项流行病学研究表明，吸烟是结核病的一个危险因素。电子烟作为传统吸烟的一种更健康的替代品而出现，但它们对结核病的影响尚不清楚。本研究旨在探讨电子烟对结核分枝杆菌吞噬作用和细胞因子产生的影响。通过将 THP-1 巨噬细胞暴露于四种电子烟蒸汽提取物来进行体外感染，并确定结核分枝杆菌的细胞内负担。通过共聚焦显微镜评估感染百分比，通过 Luminex 测定细胞因子的产生。发现 THP-1 巨噬细胞在暴露于电子烟蒸汽提取物后，其细胞内结核分枝杆菌负担减少；当使用牛分枝杆菌 GFP 株时，通过共聚焦显微镜观察到了相同的趋势。电子烟会刺激促炎细胞因子的产生。我们得出结论，电子烟会损害对结核分枝杆菌的吞噬功能和细胞因子反应。
