用于快速检测肠杆菌科细菌中广谱β-内酰胺酶产生菌的快速ESBL NP试验
Rapid ESBL NP Test for Rapid Detection of Expanded-Spectrum β-Lactamase Producers in Enterobacterales.
作者信息
Demord Anthony, Poirel Laurent, D'Emidio Fernando, Pomponio Stefano, Nordmann Patrice
机构信息
Medical and Molecular Microbiology, Faculty of Science and Medicine, University of Fribourg, Fribourg, Switzerland.
Swiss National Reference Center for Emerging Antibiotic Resistance (NARA), University of Fribourg, Fribourg, Switzerland.
出版信息
Microb Drug Resist. 2021 Aug;27(8):1131-1135. doi: 10.1089/mdr.2020.0391. Epub 2020 Dec 10.
To evaluate the biochemical Rapid ESBL NP (Liofilchem, Italy) for the rapid detection of extended-spectrum β-lactamases (ESBLs) in Enterobacterales. A total of 100 clinical gram-negative strains (40 ESBL producers with or without cephalosporinase, 43 carbapenemase producers with or without additional ESBLs, 8 AmpC-type producers, 6 penicillinase producers, and 3 non-β-lactamase producers) were tested using this colorimetric technique. The overall sensitivity and specificity of the test were found to be 93.9% and 98.5%, respectively. This test is rapid (turn-around-time of 40-45 minutes), easy to perform, and reliable for identification of ESBL producers. The Rapid ESBL NP test allows differentiation between ESBL producers on one hand, and non-ESBL producers or isolates expressing combined mechanisms of resistance on the other hand.
评估用于快速检测肠杆菌科细菌中超广谱β-内酰胺酶（ESBLs）的生化快速ESBL NP（意大利Liofilchem公司产品）。使用这种比色技术对总共100株临床革兰氏阴性菌株进行了检测（40株产ESBLs菌株，有或无头孢菌素酶；43株产碳青霉烯酶菌株，有或无额外的ESBLs；8株产AmpC型酶菌株；6株产青霉素酶菌株；3株非β-内酰胺酶产生菌）。该检测的总体敏感性和特异性分别为93.9%和98.5%。此检测快速（周转时间为40 - 45分钟）、易于操作，且对于鉴定产ESBLs菌株可靠。快速ESBL NP检测一方面能够区分产ESBLs菌株，另一方面能够区分非产ESBLs菌株或表达联合耐药机制的分离株。
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