Department of Microbiology, All India Institute of Medical Sciences, Rishikesh, 249203, India.
Department of Microbiology, All India Institute of Medical Sciences, Deoghar, 814152, India.
Eur J Med Res. 2022 Jul 16;27(1):120. doi: 10.1186/s40001-022-00752-6.
Pseudomonas aeruginosa is an adaptable bacterial pathogen that infects a variety of organs, including the respiratory tract, vascular system, urinary tract, and central nervous system, causing significant morbidity and mortality. As the primary goal of this study, we wanted to determine how pigment color production differed between clinical strains of P. aeruginosa, and whether or not that variation was associated with multidrug resistance or the ability to form biofilms. We screened in total 30.1% of yellow, 39.8% green and 30.1% of no pigment-producing P. aeruginosa strains from a total of 143 various clinical isolates. Yellow pigment-producing strains presented significant resistance to antibiotics groups, including β-lactam (91.5%), aminoglycosides (70.5%), and carbapenems (51.9%) compared to green and non-pigmented strains. Notably, 16.3% of yellow pigment-producing strains were resistant to colistin which is used as a last-resort treatment for multidrug-resistant bacteria, whereas only 2.3% of non-pigmented and 1.8% of green pigmented strains were resistant to colistin. Aside from that, yellow pigment-producing strains were frequent producers of enzymes belonging to the lactamase family, including ESBL (55.6%), MBL (55.6%), and AmpC (50%). Compared to the green groups (7.14%) and non-pigmented groups (28.5%), they had a higher frequency of efflux positive groups (64.2%). Notably, when compared to non-pigmented groups, green pigment-producing strains also displayed antibiotic susceptibility behavior similar to yellow pigment-producing strains. The majority of yellow pigment-producing strains outperformed the green and non-pigmented strains in terms of MIC levels when compared to the other two groups of strains. Despite the fact that previous studies have demonstrated a direct correlation between multidrug resistance behaviors and biofilm production, no such statistically significant association between pigment and biofilm formation was found in our investigation. Our research has demonstrated that the correlation of bacterial pigments on their susceptibility to antimicrobial agents. Yellow pigment-producing P. aeruginosa strains posed a significant problem due to the lack of alternative agents against such transformed strains, which may be associated with the development of multidrug resistance.
铜绿假单胞菌是一种适应性很强的细菌病原体，可感染多种器官，包括呼吸道、血管系统、泌尿道和中枢神经系统，导致很高的发病率和死亡率。作为本研究的主要目标，我们想确定铜绿假单胞菌临床株之间的色素颜色产生有何不同，以及这种变异是否与多药耐药性或形成生物膜的能力有关。我们总共筛选了从 143 种不同临床分离株中获得的 30.1%黄色、39.8%绿色和 30.1%无色素产生的铜绿假单胞菌菌株。与绿色和非色素产生的菌株相比，产黄色色素的菌株对包括β-内酰胺（91.5%）、氨基糖苷类（70.5%）和碳青霉烯类（51.9%）在内的抗生素组表现出显著的耐药性。值得注意的是，16.3%的产黄色色素菌株对粘菌素耐药，而粘菌素被用作多药耐药菌的最后治疗手段，而仅有 2.3%的非色素产生菌株和 1.8%的绿色色素产生菌株对粘菌素耐药。除此之外，产黄色色素的菌株频繁产生属于内酰胺酶家族的酶，包括 ESBL（55.6%）、MBL（55.6%）和 AmpC（50%）。与绿色组（7.14%）和非色素组（28.5%）相比，它们具有更高的外排阳性组频率（64.2%）。值得注意的是，与非色素产生菌株相比，绿色色素产生菌株在 MIC 水平上的抗生素敏感性行为与黄色色素产生菌株相似。与其他两组菌株相比，大多数黄色色素产生菌株在 MIC 水平上的表现优于绿色和非色素产生菌株。尽管先前的研究表明多药耐药行为与生物膜形成之间存在直接相关性，但我们的研究并未发现色素与生物膜形成之间存在统计学上显著的相关性。我们的研究表明，细菌色素与它们对抗菌药物的敏感性之间存在相关性。产黄色色素的铜绿假单胞菌菌株由于缺乏针对此类转化菌株的替代药物而构成重大问题，这可能与多药耐药性的发展有关。
