Departments of Cell Biology and Oncology, Faculty of Medicine and Dentistry, University of Alberta, Edmonton, AB, Canada.
Genome. 2021 Apr;64(4):311-325. doi: 10.1139/gen-2020-0132. Epub 2020 Dec 11.
In recent years, our perception of chromatin structure and organization in the cell nucleus has changed in fundamental ways. The 30 nm chromatin fiber has lost its status as an essential in vivo structure. Hi-C and related biochemical methods, advanced electron and super-resolved fluorescence microscopy, together with concepts from soft matter physics, have revolutionized the field. A comprehensive understanding of the structural and functional interactions that regulate cell cycle and cell type specific nuclear functions appears within reach, but it requires the integration of top-down and bottom-up approachs. In this review, I present an update on nuclear architecture studies with an emphasis on organization and the controversy regarding the physical state of chromatin in cells.
近年来，我们对细胞核中染色质结构和组织的认识已经发生了根本性的变化。30nm 染色质纤维已经失去了其作为体内基本结构的地位。Hi-C 及其相关生化方法、先进的电子和超分辨荧光显微镜，以及软物质物理的概念，彻底改变了这个领域。对调控细胞周期和细胞类型特异性核功能的结构和功能相互作用的全面理解似乎触手可及，但这需要整合自上而下和自下而上的方法。在这篇综述中，我重点介绍了核架构研究的最新进展，包括组织和关于细胞中染色质物理状态的争议。
