Institute of Parasitology, Biology Centre, Czech Academy of Sciences, Branišovská 1160/31, 370 05 České Budějovice, Czech Republic; Faculty of Science, University of South Bohemia, Branišovská 1160/31, 370 05 České Budějovice, Czech Republic.
Faculty of Science, University of South Bohemia, Branišovská 1160/31, 370 05 České Budějovice, Czech Republic.
Int J Parasitol. 2021 Apr;51(5):327-332. doi: 10.1016/j.ijpara.2020.09.010. Epub 2020 Dec 9.
We provided the first known evidence of the presence and release of extracellular vesicles in adults of important model tapeworm Hymenolepis diminuta. Two different subtypes have been observed on the surface of the worm and among the secretory products confirmed by several microscopical methods. Proteomic analysis revealed the presence of parasite-specific proteins as well as those of the host in purified extracellular vesicles. Among the protein cargo, we identified potential drug targets, vaccine candidates and H. diminuta antigens. Finally, the protein composition further revealed proteins participating in the endosomal complex required for transport-dependent biogenesis pathway.
我们首次提供了在重要的模式带绦虫 Hymenolepis diminuta 成虫中存在和释放细胞外囊泡的证据。已经通过几种显微镜方法证实，在蠕虫表面和分泌产物中观察到两种不同的亚型。蛋白质组学分析表明，在纯化的细胞外囊泡中存在寄生虫特异性蛋白以及宿主蛋白。在蛋白质载物中，我们鉴定出了潜在的药物靶点、疫苗候选物和 H. diminuta 抗原。最后，蛋白质组成进一步揭示了参与内体复合物的蛋白质，这些复合物是运输依赖性生物发生途径所必需的。
