Yang Yang, Guo Zhenli, Ye Jinting, Gao Chao-Ying, Liu Jinglin, Duan Limei
Inner Mongolia Key Laboratory for the Natural Products Chemistry and Functional Molecular Synthesis, Tongliao 028043, PR China; College of Chemistry and Material Science, Inner Mongolia University for Nationalities, Tongliao 028043, PR China.
Inner Mongolia Key Laboratory for the Natural Products Chemistry and Functional Molecular Synthesis, Tongliao 028043, PR China; College of Chemistry and Material Science, Inner Mongolia University for Nationalities, Tongliao 028043, PR China.
Spectrochim Acta A Mol Biomol Spectrosc. 2021 Mar 5;248:119238. doi: 10.1016/j.saa.2020.119238. Epub 2020 Nov 28.
Two Sulfonate substituted rhodamine hydrophilic fluorescent probes RbS1 and RbS2 were designed and synthesized for specific detection of Fe. It was found that the probe RbS2 was stronger than RbS1 in the water solubility test. Both of them displayed responses to Fe with a apparent fluorescence enhancement at 585 nm, accompanied with a distinct fluorescence change to pink. Upon addition of Fe ions (0-16 μM), the emission intensity of RbS1 and RbS2 increased to 40 and 70 fold, which exhibited a good linear relationship with the concentration of Fe. The detection limits of RbS1 and RbS2 for sensing Fe were 0.64 μM and 0.56 μM, respectively. The binding ratios of the RbS1 and RbS2 to Fe were 1:1 and the recycling ability for Fe was reasonable. RbS1 and RbS2 have been successfully applied to the determination of Fe in real water samples with satisfactory recovery and accuracy. In further living fish imaging test, the probe RbS2 was distributed into abdomen, which exhibited better fluorescence imaging ability than that of RbS1.
设计并合成了两种磺酸根取代的罗丹明类亲水性荧光探针RbS1和RbS2用于铁的特异性检测。在水溶性测试中发现探针RbS2比RbS1更强。它们对铁均有响应，在585nm处有明显的荧光增强，同时伴有明显的荧光颜色变化至粉红色。加入铁离子（0 - 16μM）后，RbS1和RbS2的发射强度分别增加到40倍和70倍，与铁浓度呈现良好的线性关系。RbS1和RbS2检测铁的检出限分别为0.64μM和0.56μM。RbS1和RbS2与铁的结合比均为1:1，且对铁具有合理的循环利用能力。RbS1和RbS2已成功应用于实际水样中铁的测定，回收率和准确度令人满意。在进一步的活鱼成像测试中，探针RbS2分布于鱼腹部，其荧光成像能力比RbS1更好。
