State Key Laboratory of Agricultural Microbiology, College of Life Science and Technology, Huazhong Agricultural University, Wuhan, 430070, China.
State Key Laboratory of Agricultural Microbiology, College of Science, Huazhong Agricultural University, Wuhan, 430070, China.
Biomaterials. 2021 Jan;268:120588. doi: 10.1016/j.biomaterials.2020.120588. Epub 2020 Dec 1.
Nitric oxide (NO) is an important biological messenger involved in the treatment of bacterial infections, but its controlled and targeted release in bacterial infections remains a major challenge. Herein, an intelligent NO nanogenerator triggered by near-infrared (NIR) light is constructed for targeted treatment of P. aeruginosa bacterial infection. Since maleimide can recognize and attach to the pilus of T4P of P. aeruginosa, we adopt this strategy to achieve the accurate release of therapeutic drugs at the infection site, i.e., after maleimide targets Gram-negative bacteria, the SNP@MOF@Au-Mal nanogenerator will release NO and generate ROS in situ from the inorganic photosensitizer gold nanoparticles under NIR irradiation to achieve synergistic antibacterial effect. In vivo experiments proved that the bacterial burden on the wound was reduced by 97.7%. Additionally, the nanogenerator was shown to promote the secretion of growth factors, which play a key role in regulating inflammation and inducing angiogenesis. This strategy has the advantage of generating a high concentration of NO in situ to promote the transfer of more NO and its derivatives (NO, ONOO) to bacteria, thereby significantly improving the antibacterial effect. The multifunctional antibacterial platform has been demonstrated as a good carrier for gas therapy because of its simple and efficient gas release performance, indicating its great potential for the treatment of drug-resistant bacterial infections.
一氧化氮（NO）是一种参与治疗细菌感染的重要生物信使，但在细菌感染中控制和靶向释放它仍然是一个主要挑战。本文构建了一种近红外（NIR）光触发的智能一氧化氮纳米发生器，用于靶向治疗铜绿假单胞菌细菌感染。由于马来酰亚胺可以识别并附着在铜绿假单胞菌 T4P 的菌毛上，我们采用这种策略来实现治疗药物在感染部位的精确释放，即在马来酰亚胺靶向革兰氏阴性菌后，SNP@MOF@Au-Mal 纳米发生器将在 NIR 照射下从无机光敏剂金纳米粒子原位释放 NO 并产生 ROS，以实现协同抗菌作用。体内实验证明，伤口处的细菌负荷减少了 97.7%。此外，该纳米发生器还被证明可以促进生长因子的分泌，生长因子在调节炎症和诱导血管生成中起着关键作用。这种策略的优势在于可以原位产生高浓度的 NO，从而促进更多的 NO 和其衍生物（NO、ONOO）向细菌转移，从而显著提高抗菌效果。由于其简单高效的气体释放性能，多功能抗菌平台已被证明是气体治疗的良好载体，表明其在治疗耐药性细菌感染方面具有巨大潜力。
