Department of Pharmaceutical Quality Assurance, SSR College of Pharmacy, Sayli Road, Silvassa, UT of Dadra and Nagar Haveli 396 230, India.
Department of Pharmaceutical Chemistry, C. U. Shah College of Pharmacy, S.N.D.T. Women's University, Santacruz (W), Mumbai 400 049, India.
Food Chem. 2021 May 30;345:128717. doi: 10.1016/j.foodchem.2020.128717. Epub 2020 Nov 25.
Charantin, a steroidal glycoside, exists as a mixture of stigmasterol glucoside (STG) and β-sitosterol glucoside (BSG) in the fruits of Momordica charantia. Charantin has anti-diabetic activity comparable to insulin. The present work discusses a method for separation of components of charantin namely STG and BSG by simple extraction technique followed by preparative HPLC. The identity of separated components was established by chromatographic as well as spectral techniques. Also reversed phase HPLC-DAD method was developed and validated for estimation of STG and BSG present in fruits of Momordica charantia. The method used C18 column (75 mm × 4.6 mm, 3.5 µm) as stationary phase and methanol: water (98:02, v/v) as mobile phase. Retention times of STG and BSG were found to be 10.707 min and 11.870 min, respectively. The validated method was applied to evaluate content of these components in different extracts and some commercial herbal formulations containing fruits of Momordica charantia.
苦瓜中的甾体糖苷，以甾醇葡萄糖苷（STG）和β-谷甾醇葡萄糖苷（BSG）的混合物形式存在。苦瓜苷具有与胰岛素相当的抗糖尿病活性。本工作讨论了一种通过简单的提取技术然后进行制备型 HPLC 分离苦瓜苷的方法，即 STG 和 BSG。通过色谱和光谱技术确定了分离成分的身份。还建立并验证了反相 HPLC-DAD 法，用于测定苦瓜果实中 STG 和 BSG 的含量。该方法使用 C18 柱（75mm×4.6mm，3.5μm）作为固定相，甲醇：水（98:02，v/v）作为流动相。STG 和 BSG 的保留时间分别为 10.707 分钟和 11.870 分钟。验证后的方法用于评估不同提取物中这些成分的含量以及一些含有苦瓜果实的商业草药配方。
