Department of Pharmaceutics, Poona College of Pharmacy, Bharati Vidyapeeth Deemed to be University, Pune, Maharashtra, India.
Department of Pharmaceutical Chemistry, Poona College of Pharmacy, Bharati Vidyapeeth Deemed to be University, Pune, Maharashtra, India.
Nat Prod Res. 2021 Mar;35(6):1035-1037. doi: 10.1080/14786419.2019.1613400. Epub 2019 Jul 2.
The dry powder of MC fruits was extracted by maceration, ultrasonication, liquid-liquid partition and soxhlation. The antidiabetic and antioxidant assays were used to screen extracts and fractions. Refluxed and liquid partitioned extracts were fractionated using petroleum ether and ethyl acetate and purified with the help of preparative HPLC to give 2 phytoconstituents M1 and M2 respectively. Compound M1 (1) was identified as charantin and Compound M2 (2) was identified as momordicinin using spectral studies. Momordicinin showed potent α-amylase inhibitory activity with IC 15.86μg/ml which was reported for the first time.
MC 果实的干粉通过浸渍、超声、液-液分配和索氏提取进行提取。使用抗糖尿病和抗氧化测定来筛选提取物和馏分。回流和液-液分配提取物分别用石油醚和乙酸乙酯进行馏分，并在制备 HPLC 的帮助下进行纯化，分别得到 2 种植物成分 M1 和 M2。通过光谱研究鉴定化合物 M1（1）为苦瓜素，化合物 M2（2）为苦瓜苷。苦瓜苷表现出很强的α-淀粉酶抑制活性，IC 15.86μg/ml，这是首次报道。
