Isolation, characterisation and investigation of antidiabetic and antioxidant activity of phytoconstituents from fruit of Linn.

The dry powder of MC fruits was extracted by maceration, ultrasonication, liquid-liquid partition and soxhlation. The antidiabetic and antioxidant assays were used to screen extracts and fractions. Refluxed and liquid partitioned extracts were fractionated using petroleum ether and ethyl acetate and purified with the help of preparative HPLC to give 2 phytoconstituents M1 and M2 respectively. Compound M1 (1) was identified as charantin and Compound M2 (2) was identified as momordicinin using spectral studies. Momordicinin showed potent α-amylase inhibitory activity with IC 15.86μg/ml which was reported for the first time.