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长链非编码 RNA GAS5 在多囊卵巢综合征中上调，并调节细胞凋亡和 IL-6 的表达。

LncRNA GAS5 is upregulated in polycystic ovary syndrome and regulates cell apoptosis and the expression of IL-6.

机构信息

Department of Reproductive medicine, The Second Affiliated Hospital of Henan, University of TCM, Henan Province, 450002, Zhengzhou City, People's Republic of China.

Henan University of TCM, 450046, Zhengzhou City, Henan Province, People's Republic of China.

出版信息

J Ovarian Res. 2020 Dec 12;13(1):145. doi: 10.1186/s13048-020-00748-y.

DOI:10.1186/s13048-020-00748-y
PMID:33308258
原文链接:https://pmc.ncbi.nlm.nih.gov/articles/PMC7733246/
Abstract

BACKGROUND

GAS5 contains a hormone response element that can induce cell apoptosis in breast cancer. It is known that cell apoptosis and hormone response play crucial roles in polycystic ovary syndrome (PCOS), indicating the potential involvement of GAS5 in PCOS. This study was performed to investigate the potential involvement of GAS5 and IL-6 (a critical player in PCOS) in PCOS.

METHODS

Research subjects of this study included 60 PCOS patients and 60 healthy controls. The expression levels of GAS5 and IL-6 in plasma of both patients and controls were measured by qPCR and ELISA, respectively. Cell transfections were performed to analyze the interaction between GAS5 and IL-6. Cell apoptosis was analyzed by cell apoptosis assay.

RESULTS

GAS5 was upregulated in plasma of PCOS patients. The expression levels of GAS5 were positively correlated with the expression levels of IL-6. Altered expression levels of GAS5 and IL-6 distinguished PCOS patients from healthy controls. In cells of a granulosa-like tumor cell line (KGN), overexpression of GAS5 led to upregulated IL-6, while silencing of GAS5 played an opposite role. Cell apoptosis analysis showed that overexpression of GAS5 significantly decreased apoptosis rate of KGN cells. Silencing of GAS5 increased the rate of KGN cell apoptosis.

CONCLUSIONS

GAS5 is upregulated in PCOS and regulates cell apoptosis and the expression of IL-6.

摘要

背景

GAS5 包含一个激素反应元件，可诱导乳腺癌细胞凋亡。已知细胞凋亡和激素反应在多囊卵巢综合征（PCOS）中起着至关重要的作用，这表明 GAS5 可能参与了 PCOS。本研究旨在探讨 GAS5 和 IL-6（PCOS 中的关键因子）在 PCOS 中的潜在作用。

方法

本研究的研究对象包括 60 名 PCOS 患者和 60 名健康对照者。通过 qPCR 和 ELISA 分别测量了患者和对照组血浆中 GAS5 和 IL-6 的表达水平。通过细胞转染分析了 GAS5 和 IL-6 之间的相互作用。通过细胞凋亡实验分析了细胞凋亡。

结果

GAS5 在 PCOS 患者的血浆中上调。GAS5 的表达水平与 IL-6 的表达水平呈正相关。GAS5 和 IL-6 表达水平的改变可将 PCOS 患者与健康对照者区分开来。在颗粒细胞样肿瘤细胞系（KGN）中，过表达 GAS5 导致 IL-6 表达上调，而沉默 GAS5 则起相反作用。细胞凋亡分析表明，过表达 GAS5 可显著降低 KGN 细胞的凋亡率。沉默 GAS5 增加了 KGN 细胞的凋亡率。

结论

GAS5 在 PCOS 中上调，并调节细胞凋亡和 IL-6 的表达。

https://cdn.ncbi.nlm.nih.gov/pmc/blobs/94f0/7733246/7d4d51ca84c6/13048_2020_748_Fig5_HTML.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/94f0/7733246/0a6796484fd2/13048_2020_748_Fig1_HTML.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/94f0/7733246/5680d2923a97/13048_2020_748_Fig2_HTML.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/94f0/7733246/bd670dd9df22/13048_2020_748_Fig3_HTML.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/94f0/7733246/c8d978c3264b/13048_2020_748_Fig4_HTML.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/94f0/7733246/7d4d51ca84c6/13048_2020_748_Fig5_HTML.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/94f0/7733246/0a6796484fd2/13048_2020_748_Fig1_HTML.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/94f0/7733246/5680d2923a97/13048_2020_748_Fig2_HTML.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/94f0/7733246/bd670dd9df22/13048_2020_748_Fig3_HTML.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/94f0/7733246/c8d978c3264b/13048_2020_748_Fig4_HTML.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/94f0/7733246/7d4d51ca84c6/13048_2020_748_Fig5_HTML.jpg

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