Department of Orthopedics, Shenzhen Second People's Hospital, Shenzhen, People's Republic of China.
J Cell Biochem. 2019 Sep;120(9):16019-16024. doi: 10.1002/jcb.28880. Epub 2019 May 8.
Excessive production of interleukin 6 (IL-6) is involved in the pathogenesis of osteoarthritis. It has been reported that long noncoding RNA (lncRNA) DILC inhibited IL-6 to regulate liver cancer stem cells. Therefore, lncRNA DILC may also participate in osteoarthritis. We found that lncRNA DILC was downregulated, while IL-6 was upregulated in plasma of osteoarthritis patients comparing to the control group. Levels of plasma lncRNA DILC and IL-6 were significantly and inversely correlated only in patients with osteoarthritis. Downregulation of lncRNA DILC effectively distinguished patients with osteoarthritis from the control group. Overexpression of lncRNA DILC resulted in inhibited IL-6 expression in chondrocytes, while treatment with exogenous IL-6 did not affect lncRNA DILC expression. However, lncRNA DILC overexpression did not affect the proliferation and apoptosis of chondrocytes. Therefore, lncRNA DILC is downregulated in osteoarthritis and regulates IL-6 expression in chondrocytes.
白细胞介素 6（IL-6）的过度产生与骨关节炎的发病机制有关。据报道，长链非编码 RNA（lncRNA）DILC 通过抑制 IL-6 来调节肝癌干细胞。因此，lncRNA DILC 也可能参与骨关节炎。我们发现，与对照组相比，骨关节炎患者的血浆中 lncRNA DILC 下调，而 IL-6 上调。仅在骨关节炎患者中，血浆 lncRNA DILC 和 IL-6 的水平呈显著负相关。lncRNA DILC 的下调可有效区分骨关节炎患者和对照组。过表达 lncRNA DILC 可抑制软骨细胞中的 IL-6 表达，而外源性 IL-6 处理不会影响 lncRNA DILC 的表达。然而，lncRNA DILC 的过表达并不影响软骨细胞的增殖和凋亡。因此，lncRNA DILC 在骨关节炎中下调，并调节软骨细胞中的 IL-6 表达。
