Carneiro Liliana P T, Ferreira Nádia S, Tavares Ana P M, Pinto Alexandra M F R, Mendes Adélio, Sales M Goreti F
BioMark, Sensor Research/UC, Department of Chemical Engineering, Faculty of Sciences and Technology, Coimbra University, Portugal; BioMark, Sensor Research/ISEP, School of Engineering, Polytechnic Institute of Porto, Portugal; CEB, Centre of Biological Engineering, Minho University, Portugal; CEFT, Department of Chemical Engineering, Faculty of Engineering, University of Porto, Portugal.
BioMark, Sensor Research/UC, Department of Chemical Engineering, Faculty of Sciences and Technology, Coimbra University, Portugal; CEB, Centre of Biological Engineering, Minho University, Portugal.
Biosens Bioelectron. 2021 Mar 1;175:112877. doi: 10.1016/j.bios.2020.112877. Epub 2020 Dec 5.
This work describes an electrochemical sensor with a biomimetic plastic antibody film for carcinoembryonic antigen (CEA, an important biomarker in colorectal cancer), integrated in the electrical circuit of a direct methanol fuel cell (DMFC), working in passive mode and used herein as power supply and signal transducer. In detail, the sensing layer for CEA consisted of a Fluorine-doped Tin Oxide (FTO) conductive glass substrate - connected to the negative pole side of the DMFC - with a conductive poly (3,4-ethylenedioxythiophene) (PEDOT) layer and a polypyrrol (PPy) molecularly-imprinted polymer (MIP), assembled in-situ. This sensing element is then closed using a cover FTO-glass, hold in place with a clip, connected to the positive side of the DMFC. When compared with control DMFCs, the power curves of DMFC/Sensor integrated system showed decreased power values due to the MIP layer interfaced in the electrical circuit, also displaying high stability signals. The DMFC/Sensor was further calibrated at room temperature, in different medium (buffer, a synthetic physiological fluid model and Cormay® serum), showing linear responses over a wide concentration range, with a limit of detection of 0.08 ng/mL. The DMFC/Sensor presented sensitive data, with linear responses from 0.1 ng/mL to 100 μg/mL and operating well in the presence of human serum. Overall, the results obtained evidenced the possibility of using a DMFC as a transducing element in an electrochemical sensor, confirming the sensitive and selective readings of the bio (sensing) imprinted film. This integration paves the way towards fully autonomous electrochemical devices, in which the integration of the sensor inside the fuel cell may be a subsequent direction.
这项工作描述了一种带有用于癌胚抗原（CEA，结直肠癌中的一种重要生物标志物）的仿生塑料抗体膜的电化学传感器，该传感器集成在直接甲醇燃料电池（DMFC）的电路中，以被动模式工作，在此用作电源和信号转换器。详细而言，用于CEA的传感层由连接到DMFC负极侧的氟掺杂氧化锡（FTO）导电玻璃基板组成，其上原位组装有导电聚（3,4 - 乙撑二氧噻吩）（PEDOT）层和聚吡咯（PPy）分子印迹聚合物（MIP）。然后使用覆盖的FTO玻璃封闭该传感元件，用夹子固定到位，并连接到DMFC的正极。与对照DMFC相比，DMFC/传感器集成系统的功率曲线显示由于电路中接入的MIP层导致功率值降低，同时也显示出高稳定性信号。DMFC/传感器在室温下于不同介质（缓冲液、合成生理流体模型和科美血清）中进一步校准，在宽浓度范围内呈现线性响应，检测限为0.08 ng/mL。DMFC/传感器呈现出灵敏的数据，在0.1 ng/mL至100 μg/mL范围内具有线性响应，并且在人血清存在下运行良好。总体而言，所获得的结果证明了将DMFC用作电化学传感器中的转换元件的可能性，证实了生物（传感）印迹膜的灵敏和选择性读数。这种集成朝着完全自主的电化学装置迈出了一步，其中将传感器集成在燃料电池内部可能是后续的一个方向。
