Department of Radiology, IVR center, Nara Medical University, Kashihara, Japan.
Department of Radiology, IVR center, Nara Medical University, Kashihara, Japan.
J Vasc Interv Radiol. 2021 Mar;32(3):376-383. doi: 10.1016/j.jvir.2020.10.017. Epub 2020 Dec 9.
To assess the angiographic findings and the effects of transcatheter arterial embolization on physical activity and histopathology using a frozen shoulder rat model.
First, the angiographic and histopathologic findings of rats in which the shoulder was immobilized with molding plaster for 6 weeks (n = 4) were compared to control rats with normal non-immobilized shoulders (n = 4). Next, a total of 16 frozen shoulder rats were divided into 2 groups. In the transcatheter arterial embolization group (n = 8), imipenem/cilastatin was injected into the left thoracoacromial artery. The changes of physical activity before and after procedures were evaluated and compared with a saline-injected control group (n = 8). Histopathologic findings were also compared between the 2 groups.
Angiography revealed abnormal shoulder staining in all of the rats with a frozen shoulder. On histopathology, the numbers of microvessels and mononuclear inflammatory cells in the synovial membrane of the joint capsule were significantly higher compared with the control rats (both P = .03). In the transcatheter arterial embolization group, the running distance and speed were improved (P = .03 and P = .01, respectively), whereas there were no significant differences in the control group. The number of microvessels and mononuclear inflammatory cells in the transcatheter arterial embolization group were significantly lower than the control group (P = .002 and P = .001, respectively).
The rat frozen shoulder model revealed the development of neovascularization. Transcatheter arterial embolization decreased the number of blood vessels and inflammatory changes in the frozen shoulder and increased the moving distance and speed of the rats.
使用冻结肩大鼠模型评估血管造影表现和经导管动脉栓塞对体力活动和组织病理学的影响。
首先，比较了用模塑石膏固定肩部 6 周的大鼠（n=4）和具有正常非固定肩部的对照组大鼠（n=4）的血管造影和组织病理学发现。接下来，总共 16 只冻结肩大鼠分为 2 组。在经导管动脉栓塞组（n=8）中，将亚胺培南/西司他丁注入左胸锁乳突肌动脉。评估并比较了治疗前后的体力活动变化，并与生理盐水注射对照组（n=8）进行了比较。还比较了两组的组织病理学发现。
血管造影显示所有冻结肩大鼠均出现肩部异常染色。组织病理学检查显示，关节囊滑膜中的微血管和单核炎性细胞数量明显高于对照组（均 P=0.03）。在经导管动脉栓塞组中，跑动距离和速度均有所改善（P=0.03 和 P=0.01），而对照组无明显差异。经导管动脉栓塞组的微血管和单核炎性细胞数量明显低于对照组（P=0.002 和 P=0.001）。
大鼠冻结肩模型显示出新生血管的发展。经导管动脉栓塞减少了冻结肩的血管数量和炎症变化，增加了大鼠的运动距离和速度。
