基于金纳米颗粒聚集的暗场显微镜观察用于抗体检测的蛋白功能化金纳米颗粒
Protein-Functionalized Gold Nanoparticles for Antibody Detection Using the Darkfield Microscopic Observation of Nanoparticle Aggregation.
机构信息
Department of Chemistry and Biology, Graduate School of Science and Engineering, Ehime University, 2-5 Bunkyo, Matsuyama, Ehime, 790-8577, Japan.
Department of Applied Chemistry, Graduate School of Engineering, Kyushu University, 744 Motooka, Fukuoka, 819-0395, Japan.
出版信息
Anal Sci. 2021 Mar 10;37(3):507-511. doi: 10.2116/analsci.20SCP12. Epub 2020 Dec 11.
Gold nanoparticles (AuNPs) are commonly used in biosensing applications. In this study, AuNPs were synthesized by using reduced bovine serum albumin (rBSA) as the reducing agent. The rBSA conjugated with AuNPs via Au-Sulfur interactions to form rBSA-functionalized AuNPs (rBSA-AuNPs). The interaction of the rBSA moieties on the rBSA-AuNP surface with an anti-BSA antibody (anti-BSA) led to AuNP aggregation, which enabled the successful detection of anti-BSA at a concentration as low as 20 nM through darkfield microscopy (DFM). This study demonstrates the potential applications of protein-functionalized AuNPs in the bioanalysis of substances through DFM.
金纳米粒子（AuNPs）常用于生物传感应用。在本研究中，使用还原牛血清白蛋白（rBSA）作为还原剂合成了 AuNPs。rBSA 通过 Au-S 相互作用与 AuNPs 结合，形成 rBSA 功能化的 AuNPs（rBSA-AuNPs）。rBSA-AuNP 表面的 rBSA 部分与抗 BSA 抗体（anti-BSA）相互作用导致 AuNP 聚集，通过暗场显微镜（DFM）成功检测到浓度低至 20 nM 的抗 BSA。本研究证明了通过 DFM 对物质进行生物分析时，蛋白质功能化 AuNPs 的潜在应用。
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