不同尺寸金纳米颗粒对牛血清白蛋白的表面增强拉曼散射研究

Surface-enhanced Raman scattering investigation of bovine serum albumin by Au nanoparticles with different sizes.

作者信息

Xiaodan Wei, Dawei Zheng, Ping Zhang, Taifeng Lin, Huiqin Wang, Yongwei Zhu

机构信息

1 College of Life science and Bioengineering, Beijing University of Technology, Beijing, China.

2 Department of State-owned Assets and Laboratory Management, Beijing University of Technology, Beijing, China.

出版信息

J Appl Biomater Funct Mater. 2018 Jan;16(1_suppl):157-162. doi: 10.1177/2280800017753055.

DOI:10.1177/2280800017753055

PMID:29618248

Abstract

BACKGROUND

Surface-enhanced Raman scattering (SERS) has become a useful spectroscopic tool for studying biomolecule structures. The main types of plasmonic substrates used in biological systems are Au nanoparticles (AuNPs), whose surface plasmon resonance depends on the nanoparticle size, morphology, particle interspace, and so on.

METHODS

In this study, AuNP colloids with different sizes were synthesized and used as the sensors to probe SERS signals of different biomarkers and biomolecules.

RESULTS

The results showed that an AuNP colloid of ~50 nm had excellent SERS effects in probing various molecules, and could be preserved for about 3 months with excellent repeatability and reproducibility (RSD <5%) in terms of the probed signal intensity (rhodamine 6G and crystal violet). Meanwhile, the fabricated AuNPs were applied to study the SERS signals and structural information of bovine serum albumin (BSA) in aqueous solution. It was found that SERS could rapidly provide the structural information and vibration characteristics of BSA.

CONCLUSION

It was concluded that biocompatible AuNP colloid may be a promising biosensor in the rapid and label-free detection of biological systems.

摘要

背景

表面增强拉曼散射（SERS）已成为研究生物分子结构的一种有用的光谱工具。生物系统中使用的主要等离子体基底类型是金纳米颗粒（AuNPs），其表面等离子体共振取决于纳米颗粒的大小、形态、颗粒间距等。

方法

在本研究中，合成了不同尺寸的AuNP胶体，并用作传感器来探测不同生物标志物和生物分子的SERS信号。

结果

结果表明，约50 nm的AuNP胶体在探测各种分子时具有优异的SERS效应，并且就探测信号强度（罗丹明6G和结晶紫）而言，可保存约3个月，具有出色的重复性和再现性（RSD<5%）。同时，将制备的AuNPs应用于研究水溶液中牛血清白蛋白（BSA）的SERS信号和结构信息。发现SERS可以快速提供BSA的结构信息和振动特征。