Department of Animal Science, University of Nebraska-Lincoln, Animal Science Complex, 3940 Fair St., Lincoln, NE, 68583-0908, USA.
Department of Food Science and Technology, Food Innovation Center, University of Nebraska-Lincoln, 1901 N 21st St., Lincoln, NE, 68588-6205, USA.
Commun Biol. 2020 Dec 11;3(1):760. doi: 10.1038/s42003-020-01477-0.
The majority of microbiome studies focused on understanding mechanistic relationships between the host and the microbiota have used mice and other rodents as the model of choice. However, the domestic pig is a relevant model that is currently underutilized for human microbiome investigations. In this study, we performed a direct comparison of the engraftment of fecal bacterial communities from human donors between human microbiota-associated (HMA) piglet and mouse models under identical dietary conditions. Analysis of 16S rRNA genes using amplicon sequence variants (ASVs) revealed that with the exception of early microbiota from infants, the more mature microbiotas tested established better in the HMA piglets compared to HMA mice. Of interest was the greater transplantation success of members belonging to phylum Firmicutes in the HMA piglets compared to the HMA mice. Together, these results provide evidence for the HMA piglet model potentially being more broadly applicable for donors with more mature microbiotas while the HMA mouse model might be more relevant for developing microbiotas such as those of infants. This study also emphasizes the necessity to exercise caution in extrapolating findings from HMA animals to humans, since up to 28% of taxa from some donors failed to colonize either model.
大多数专注于理解宿主与微生物组之间机制关系的微生物组研究都使用了小鼠和其他啮齿动物作为首选模型。然而，家猪是一种相关的模型，目前在人类微生物组研究中未得到充分利用。在这项研究中，我们在相同的饮食条件下，直接比较了来自人类供体的粪便细菌群落在与人共生（HMA）仔猪和小鼠模型中的定植情况。使用扩增子序列变异（ASV）对 16S rRNA 基因进行分析表明，除了来自婴儿的早期微生物群外，在 HMA 仔猪中，与 HMA 小鼠相比，测试的更成熟的微生物群建立得更好。有趣的是，与 HMA 小鼠相比，厚壁菌门成员在 HMA 仔猪中的移植成功率更高。这些结果共同为 HMA 仔猪模型可能更广泛地适用于具有更成熟微生物群的供体提供了证据，而 HMA 小鼠模型可能更适用于开发微生物群，如婴儿的微生物群。这项研究还强调了从 HMA 动物推断到人类时需要谨慎，因为高达 28%的某些供体的分类群未能定植于任何一种模型中。
