Valipour AbdolMajid, Heidari Behrooz, Asghari S Mohsen, Balalaie Saeed, Rabouti Hanieh, Omidian Navid
Department of Biology, Faculty of Science, University of Guilan, Rasht, Iran.
Institute of Biochemistry and Biophysics, University of Tehran, Tehran, Iran.
J Fish Biol. 2021 Apr;98(4):1137-1143. doi: 10.1111/jfb.14645. Epub 2021 Jan 3.
Despite several studies on fish hormone therapy, finding new candidates may provide more reproductive efficiency in artificial propagation. Kisspeptins, being upstream of the hypothalamic-pituitary-gonadal axis, appear to play a key role in the reproduction process. In the present study, the effect of different variants of kisspeptide, including goldfish (Carassius auratus) kiss1 kisspeptin (Kiss1), human kisspeptin (Hkiss), and their combination (Kiss1 + H), on the reproductive indices of goldfish broodstock in comparison to Ovaprim (a typical synthetic Gnrh hormone) was investigated. Peptides (Kiss1 and Hkiss) were synthesized using a solid-phase synthesis approach. Kiss1 and Hkiss were injected at a dose of 100 μg kg body weight, blood samples were taken 6 h after injection and sex hormones (E2, Dhp, and 11-Kt), gonadotropins (Lh and Fsh), cortisol and reproductive indices (fecundity, fertilization and hatching percentage) were measured. The results showed a significant increase of plasma sex hormones and gonadotropins in fish treated with kisspeptins. In addition, the cortisol and lipoprotein lipase in Kiss1, Hkiss and Kiss1 + H were remarkably increased compared to Ovaprim. In conclusion, kisspeptins could be a more suitable candidate than Ovaprim for accelerating and synchronizing oocyte maturation in the fisheries industry.
尽管对鱼类激素疗法已开展了多项研究，但寻找新的候选物质可能会在人工繁殖中提高繁殖效率。 kisspeptins位于下丘脑-垂体-性腺轴的上游，似乎在繁殖过程中起关键作用。在本研究中，研究了不同变体的吻肽，包括金鱼（Carassius auratus）吻1吻肽（Kiss1）、人吻肽（Hkiss）及其组合（Kiss1 + H），与Ovaprim（一种典型的合成Gnrh激素）相比，对金鱼亲鱼繁殖指标的影响。肽（Kiss1和Hkiss）采用固相合成方法合成。以100μg/kg体重的剂量注射Kiss1和Hkiss，注射6小时后采集血样，测定性激素（E2、Dhp和11-Kt）、促性腺激素（Lh和Fsh）、皮质醇和繁殖指标（产卵量、受精率和孵化率）。结果显示，用吻肽处理的鱼血浆性激素和促性腺激素显著增加。此外，与Ovaprim相比，Kiss1、Hkiss和Kiss1 + H中的皮质醇和脂蛋白脂肪酶显著增加。总之，在渔业中，吻肽可能是比Ovaprim更适合加速和同步卵母细胞成熟的候选物质。
