二氯甲烷在小鼠骨髓微核试验中的无活性。

Inactivity of methylene chloride in the mouse bone marrow micronucleus assay.

作者信息

Sheldon T, Richardson C R, Elliott B M

机构信息

Imperial Chemical Industries, Central Toxicology Laboratory, Macclesfield, Cheshire, UK.

出版信息

Mutagenesis. 1987 Jan;2(1):57-9. doi: 10.1093/mutage/2.1.57.

DOI:10.1093/mutage/2.1.57

PMID:3331695

Abstract

Methylene chloride (MC) has been evaluated for its ability to induce micronucleated polychromatic erythrocytes (MPEs) in the bone marrow of treated mice. Groups of five male and five female C57BL/6J/Alpk mice were exposed, by gavage, to doses of 4000, 2500 and 1250 mg/kg MC in corn oil, the highest dose-level being selected to be the maximum tolerated dose. Bone marrow samples were taken 24, 36, 48 and 72 h after dosing. No significant increases in the incidence of MPEs over controls were observed for any of the test groups, and it is concluded that MC is not clastogenic in this assay.

摘要

已对二氯甲烷（MC）在经处理小鼠骨髓中诱导微核多染红细胞（MPEs）的能力进行了评估。将每组五只雄性和五只雌性C57BL/6J/Alpk小鼠通过灌胃给予玉米油中剂量分别为4000、2500和1250 mg/kg的MC，最高剂量水平被选定为最大耐受剂量。给药后24、36、48和72小时采集骨髓样本。在任何测试组中，均未观察到MPEs发生率相对于对照组有显著增加，由此得出结论，在该试验中MC无致断裂作用。