Montes de Oca-Luna R, Leal-Garza C H, Baca-Sevilla S, Garza-Chapa R
Mutat Res. 1984 Nov-Dec;141(3-4):183-7. doi: 10.1016/0165-7992(84)90094-0.
Using the micronucleus test to evaluate the mutagenic effect of 5,5-diphenylhydantoin (DPH) on bone marrow polychromatic erythrocytes, male Balb-C mice were treated with the drug in single and multiple injection tests. A significant increase in the frequency of micronucleated polychromatic erythrocytes (MPE), P less than 0.05, was found when the mice received a single injection of DPH at doses of 0.5 and 1.0 mg/kg, and this frequency did not increase at higher doses. When mice were treated 3 times, at 24-h intervals, with 1.0 mg/kg of DPH, a significant increase in MPE was also observed (P less than 0.05) but this was lower than when they received a single injection of the same dose. A cytotoxic effect of NaOH, 0.1 N, which was used as solvent, was also observed either when alone or when DPH (1.0 mg/kg) was injected 3 times. This effect was comparable to the one produced by mitomycin C (MMC) at a dose of 0.5 mg/kg.
利用微核试验评估5,5-二苯基乙内酰脲（DPH）对雄性Balb-C小鼠骨髓嗜多染红细胞的诱变作用，在单次和多次注射试验中用该药物处理小鼠。当小鼠接受0.5和1.0mg/kg剂量的单次DPH注射时，发现微核嗜多染红细胞（MPE）频率显著增加（P<0.05），且在更高剂量下该频率未增加。当小鼠每隔24小时用1.0mg/kg的DPH处理3次时，也观察到MPE显著增加（P<0.05），但低于单次注射相同剂量时的情况。还观察到用作溶剂的0.1N氢氧化钠单独使用或与DPH（1.0mg/kg）一起注射3次时具有细胞毒性作用。这种作用与0.5mg/kg剂量的丝裂霉素C（MMC）产生的作用相当。
