Chongqing Key Laboratory of Optical Fiber Sensor and Photoelectric Detection, Chongqing University of Technology, Chongqing 400054, China.
School of Pharmacy and Bioengineering, Chongqing University of Technology, Chongqing 400054, China.
Sensors (Basel). 2023 May 31;23(11):5218. doi: 10.3390/s23115218.
() is a zoonotic parasite that is widely distributed and seriously endangers public health and human health. Therefore, accurate and effective detection of is crucial. This study proposes a microfluidic biosensor using a thin-core microfiber (TCMF) coated with molybdenum disulfide (MoS) for immune detection of . The single-mode fiber was fused with the thin-core fiber, and the TCMF was obtained by arc discharging and flame heating. In order to avoid interference and protect the sensing structure, the TCMF was encapsulated in the microfluidic chip. MoS and antigen were modified on the surface of TCMF for the immune detection of . Experimental results showed that the detection range of the proposed biosensor for monoclonal antibody solutions was 1 pg/mL to 10 ng/mL with sensitivity of 3.358 nm/log(mg/mL); the detection of limit was calculated to be 87 fg/mL through the Langmuir model; the dissociation constant and the affinity constant were calculated to be about 5.79 × 10 M and 1.727 × 10 M, respectively. The specificity and clinical characteristics of the biosensor was explored. The rabies virus, pseudorabies virus, and serum were used to confirm the excellent specificity and clinical characteristics of the biosensor, indicating that the proposed biosensor has great application potential in the biomedical field.
（）是一种人畜共患寄生虫，分布广泛，严重危害公众健康和人类健康。因此，准确有效地检测（）至关重要。本研究提出了一种使用涂覆二硫化钼（MoS）的薄壁光纤（TCMF）的微流控生物传感器，用于（）的免疫检测。单模光纤与薄壁光纤融合，通过电弧放电和火焰加热获得 TCMF。为了避免干扰和保护传感结构，将 TCMF 封装在微流控芯片中。MoS 和（）抗原被修饰在 TCMF 的表面，用于（）的免疫检测。实验结果表明，该生物传感器对单克隆抗体溶液的检测范围为 1pg/mL 至 10ng/mL，灵敏度为 3.358nm/log(mg/mL)；通过 Langmuir 模型计算得出检测限为 87fg/mL；解离常数和亲和常数分别约为 5.79×10M 和 1.727×10M。还探索了该生物传感器的特异性和临床特征。使用狂犬病病毒、伪狂犬病病毒和（）血清来确认该生物传感器具有优异的特异性和临床特征，表明所提出的生物传感器在生物医学领域具有巨大的应用潜力。
