National Centre for Vector Entomology, Institute of Parasitology, University of Zürich, Zürich, Switzerland.
Section of Epidemiology, Vetsuisse Faculty, University of Zürich, Zürich, Switzerland.
Med Vet Entomol. 2021 Sep;35(3):324-332. doi: 10.1111/mve.12500. Epub 2020 Dec 15.
Biting midges (Diptera: Ceratopogonidae) are arthropods of veterinary importance since they can transmit pathogens and cause severe allergic dermatitis in horses. Very little is known about the species at higher altitudes and their seasonal dynamics. In this work, adult Culicoides were collected with Onderstepoort UV-light suction traps (OVI) from June to September 2016 at two areas situated at around 1600 m asl (pre-alpine area I, 2 farms) and 2030 m asl (pre-alpine area II, 1 farm) in the Canton of Grisons (south-east Switzerland). Overall, 17 049 Culicoides were collected, including 871 parous females. A total of 50 individuals/trap/night (n = 1050) were identified to species (17 species) by matrix-assisted laser desorption/ionization time-of-flight mass spectrometry (MALDI-TOF MS) or by polymerase chain reaction (PCR) and sequencing. The remaining 15 128 Culicoides were classified to species groups' level. Culicoides obsoletus (Meigen, 1818), a multivoltine species, was mainly present at 1600 m asl, whereas at high altitudes (2030 m asl), C. grisescens Edwards, 1939 I&II were the most abundant species. In particular, C. grisescens II, which seems to be univoltine, occurred later in the season but significantly increasing over time. Species diversity was higher at pre-alpine I area (n = 16 species) compared to pre-alpine II (n = 10 species).
吸血蠓（双翅目：蠓科）是一种对兽医具有重要意义的节肢动物，因为它们可以传播病原体并导致马严重的过敏性皮炎。对于高海拔地区的物种及其季节性动态，人们知之甚少。在这项工作中，从 2016 年 6 月到 9 月，使用 Onderstepoort 紫外线抽吸陷阱（OVI）在海拔约 1600 米（前阿尔卑斯地区 I，2 个农场）和 2030 米（前阿尔卑斯地区 II，1 个农场）的两个地区收集了成虫库蠓。总共收集了 17049 只库蠓，包括 871 只已产卵的雌性。总共对 50 个个体/陷阱/夜（n=1050）进行了物种鉴定（17 个物种），方法是基质辅助激光解吸/电离飞行时间质谱（MALDI-TOF MS）或聚合酶链反应（PCR）和测序。其余 15128 只库蠓被归类为种组水平。多化性物种 C. obsoletus（Meigen，1818）主要存在于 1600 米海拔，而在高海拔（2030 米海拔），C. grisescens Edwards，1939 I&II 是最丰富的物种。特别是似乎单化性的 C. grisescens II 出现较晚，但随着时间的推移，其数量显著增加。前阿尔卑斯地区 I（n=16 种）的物种多样性高于前阿尔卑斯地区 II（n=10 种）。
