Esposito F, Lombardi S, Toure Y T, Zavala F, Coluzzi M
Dipartimento di Biologia Cellulare, Università di Camerino, Italia.
Parassitologia. 1986 Apr;28(1):69-77.
Samples of indoor-resting Anopheles gambiae s.1. from Mali and Burkina Faso (West Africa) were processed in order to compare Plasmodium falciparum sporozoite rates obtained by immunoradiometric assay (IRMA) with circumsporozoite (CS) monoclonal antibody and by microscope examination of salivary glands. The immunological method provided sporozoite rates always higher than those obtained by microscope examination. This result does not appear to be related to cross-reactions involving non-sporozoite antigens. A small fraction of IRMA-positive mosquitoes is necessarily negative by microscope, since these mosquitoes actually contain the CS antigen only in the abdomen, presumably in connection with the presence of fully mature oocysts. However, the frequency of these mosquitoes cannot explain in itself an average ratio of 1:2 between microscope and IRMA sporozoite rates. A more important source of difference appears to depend on the detection of positive mosquitoes with low sporozoite numbers which remain more frequently undetected by microscope examination. Failure of salivary gland penetration by sporozoites is also considered as a possible source of discrepancy between the two methods.
对来自马里和布基纳法索（西非）的室内栖息冈比亚按蚊（Anopheles gambiae s.l.）样本进行了处理，以便比较通过免疫放射分析（IRMA）使用环子孢子（CS）单克隆抗体获得的恶性疟原虫子孢子率和通过显微镜检查唾液腺获得的子孢子率。免疫方法提供的子孢子率总是高于通过显微镜检查获得的子孢子率。这一结果似乎与涉及非子孢子抗原的交叉反应无关。一小部分IRMA阳性蚊子通过显微镜检查必然为阴性，因为这些蚊子实际上仅在腹部含有CS抗原，推测这与完全成熟的卵囊的存在有关。然而，这些蚊子的频率本身并不能解释显微镜检查和IRMA子孢子率之间平均为1:2的比例。一个更重要的差异来源似乎取决于对低子孢子数量阳性蚊子的检测，这些蚊子通过显微镜检查更频繁地未被检测到。子孢子未能穿透唾液腺也被认为是两种方法之间差异的一个可能来源。
