Shimamura Yuko, Inagaki Ryo, Honda Hiroshi, Masuda Shuichi
School of Food and Nutritional Sciences, University of Shizuoka, 52-1 Yada, Suruga-ku, Shizuoka 422-8526, Japan.
KAO Corporation, R&D Safety Science Research, 2606 Akabane, Ichikai-Machi, Haga-Gun, Tochigi 321-3497, Japan.
Toxics. 2020 Dec 13;8(4):119. doi: 10.3390/toxics8040119.
Glycidyl fatty acid esters (GE) are constituents of edible oils and fats, and are converted into glycidol, a genotoxic substance, in vivo. -(2,3-dihydroxypropyl)valine (diHOPrVal), a hemoglobin adduct of glycidol, is used as a biomarker of glycidol and GE exposure. However, high background levels of diHOPrVal are not explained by daily dietary exposure to glycidol and GE. In the present study, several glycidol-related chemicals (glycidol, (±)-3-chloro-1,2-propanediol, glycidyl oleate, epichlorohydrin, propylene oxide, 1-bromopropane, allyl alcohol, fructose, and glyceraldehyde) that might be precursors of diHOPrVal, were administered to mice, and diHOPrVal formation from each substance was examined with LC-MS/MS. DiHOPrVal was detected in animals treated with glycidol and glycidyl oleate but not in mice treated with other chemicals (3-MCPD, epichlorohydrin, propylene oxide, 1-bromopropane, allyl alcohol, fructose, and glyceraldehyde). The amount of diHOPrVal per administered dose produced from other chemicals was negligible compared to the amounts associated with dietary glycidol and GE. The present study provides important knowledge for exploring other sources for internal exposure to glycidol.
缩水甘油脂肪酸酯（GE）是食用油脂的成分，在体内可转化为具有遗传毒性的物质缩水甘油。-（2,3-二羟基丙基）缬氨酸（diHOPrVal）是缩水甘油的血红蛋白加合物，用作缩水甘油和GE暴露的生物标志物。然而，日常饮食中缩水甘油和GE的暴露并不能解释diHOPrVal的高背景水平。在本研究中，将几种可能是diHOPrVal前体的与缩水甘油相关的化学物质（缩水甘油、（±）-3-氯-1,2-丙二醇、油酸缩水甘油酯、环氧氯丙烷、环氧丙烷、1-溴丙烷、烯丙醇、果糖和甘油醛）给予小鼠，并用液相色谱-串联质谱法检测每种物质形成diHOPrVal的情况。在用缩水甘油和油酸缩水甘油酯处理的动物中检测到了diHOPrVal，但在用其他化学物质（3-氯-1,2-丙二醇、环氧氯丙烷、环氧丙烷、1-溴丙烷、烯丙醇、果糖和甘油醛）处理的小鼠中未检测到。与饮食中缩水甘油和GE产生的量相比，其他化学物质每给药剂量产生的diHOPrVal量可忽略不计。本研究为探索缩水甘油的其他内暴露来源提供了重要知识。
