Tochigi Research Laboratories, Kao Corporation, 2606 Akabane, Ichikai-Machi, Haga-Gun, Tochigi 321-3497, Japan.
Food Chem Toxicol. 2011 Oct;49(10):2536-40. doi: 10.1016/j.fct.2011.06.057. Epub 2011 Jun 29.
Hemoglobin (Hb) adducts are frequently used to address and/or monitor exposure to reactive chemicals. Glycidol (G), a known animal carcinogen, has been reported to form Hb adducts. Here, we measure G adduct levels in humans who daily ingest DAG oil, an edible oil consisting mainly of diacylglycerol. Since DAG oil contains a small amount of glycidol fatty acid esters (GEs), possible exposure to G released from GEs has been raised as a possible concern. For measurement of Hb adducts, we employed the N-alkyl Edman method reported by Landin et al. (1996) using gas chromatography-tandem mass spectrometry with minor modifications to detect G-Hb adducts as N-(2,3-dihydroxy-propyl)valine (diHOPrVal). Blood samples were collected from 7 DAG oil users and 6 non-users, and then G-Hb adduct levels were measured. G-Hb adducts were detected in all samples. The average level of diHOPrVal was 3.5±1.9pmol/g globin in the DAG oil users and 7.1±3.1pmol/g globin in the non-users. We conclude that there is no increased exposure to G in individuals who daily ingest DAG oil.
血红蛋白（Hb）加合物常用于评估和/或监测对反应性化学物质的暴露情况。缩水甘油（G）是一种已知的动物致癌物，已被报道会形成 Hb 加合物。在这里，我们测量了每天摄入富含二酰基甘油（DAG）的食用油的人群中的 G 加合物水平。由于 DAG 油中含有少量的缩水甘油脂肪酸酯（GE），因此可能会接触到从 GEs 释放的 G 已被提出作为一种可能的关注点。为了测量 Hb 加合物，我们采用了 Landin 等人（1996 年）报道的 N-烷基 Edman 方法（1996 年），使用气相色谱-串联质谱法进行了轻微修改，以检测 N-（2,3-二羟丙基）缬氨酸（diHOPrVal）作为 G-Hb 加合物。从 7 名 DAG 油使用者和 6 名非使用者采集了血液样本，然后测量了 G-Hb 加合物水平。所有样本中均检测到 G-Hb 加合物。DAG 油使用者的 diHOPrVal 平均水平为 3.5±1.9pmol/g 球蛋白，而非使用者的 diHOPrVal 平均水平为 7.1±3.1pmol/g 球蛋白。我们得出结论，每天摄入 DAG 油的个体没有增加 G 的暴露。
